The interaction between eukaryotic initiation factor 1A and eIF5 retains eIF1 within scanning preinitiation complexes
dc.citation.doi | doi:10.1021/bi4009775 | en_US |
dc.citation.epage | 9518 | en_US |
dc.citation.issue | 52 | en_US |
dc.citation.jtitle | Biochemistry | en_US |
dc.citation.spage | 9510 | en_US |
dc.citation.volume | 52 | en_US |
dc.contributor.author | Luna, Rafael E. | |
dc.contributor.author | Arthanari, Haribabu | |
dc.contributor.author | Hiraishi, Hiroyuki | |
dc.contributor.author | Akabayov, Barak | |
dc.contributor.author | Tang, Leiming | |
dc.contributor.author | Cox, Christian | |
dc.contributor.author | Markus, Michelle A. | |
dc.contributor.author | Luna, Lunet E. | |
dc.contributor.author | Ikeda, Yuka | |
dc.contributor.author | Watanabe, Ryosuke | |
dc.contributor.author | Bedoya, Edward | |
dc.contributor.author | Yu, Cathy | |
dc.contributor.author | Alikhan, Shums | |
dc.contributor.author | Wagner, Gerhard | |
dc.contributor.author | Asano, Katsura | |
dc.contributor.authoreid | hiraishi | en_US |
dc.contributor.authoreid | kasano | en_US |
dc.date.accessioned | 2014-05-06T19:41:43Z | |
dc.date.available | 2014-05-06T19:41:43Z | |
dc.date.issued | 2014-05-06 | |
dc.date.published | 2013 | en_US |
dc.description.abstract | Scanning of the mRNA transcript by the preinitiation complex (PIC) requires a panel of eukaryotic initiation factors including eIF1 and eIF1A, the main transducers of stringent AUG selection. eIF1A plays an important role in start codon recognition; however, its molecular contacts with eIF5 are unknown. Using NMR, we unveil eIF1A’s binding surface on the carboxyl-terminal domain of eIF5 (eIF5-CTD). We validated this interaction by observing that eIF1A does not bind to an eIF5-CTD mutant, altering the revealed eIF1A-interaction site. We also found that the interaction between eIF1A:eIF5-CTD is conserved between human and yeast. Using GST pull down assays of purified proteins, we showed that the N-terminal tail (NTT) of eIF1A mediates the interaction with eIF5-CTD and eIF1. Genetic evidence indicates that overexpressing eIF1 or eIF5 suppresses the slow growth phenotype of eIF1A-NTT mutants. These results suggest that the eIF1A:eIF5-CTD interaction during scanning PICs contributes to the maintenance of eIF1 within the open PIC. | en_US |
dc.identifier.uri | http://hdl.handle.net/2097/17685 | |
dc.language.iso | en_US | en_US |
dc.relation.uri | http://pubs.acs.org/doi/full/10.1021/bi4009775 | en_US |
dc.rights | Permission to archive granted by American Chemical Society, March 20, 2014. This document is the unedited Author's version of a Submitted Work that was subsequently accepted for publication in Biochemistry, copyright © American Chemical Society after peer review. To access the final edited and published work see http://pubs.acs.org/doi/full/10.1021/bi4009775 | en_US |
dc.subject | Preinitiation complex | en_US |
dc.subject | Eukaryotic initiation factors | en_US |
dc.subject | eIF1 | en_US |
dc.subject | eIF1A | en_US |
dc.subject | eIF5 | en_US |
dc.title | The interaction between eukaryotic initiation factor 1A and eIF5 retains eIF1 within scanning preinitiation complexes | en_US |
dc.type | Article (author version) | en_US |