Immobilization of ß-Galactosidase in Adsorptive Membranes for the Continuous Production of Galacto-Oligosaccharides from Lactose

dc.citation.doi10.2174/1874256400701010017en_US
dc.citation.epage22en_US
dc.citation.jtitleThe Open Food Science Journalen_US
dc.citation.spage17en_US
dc.citation.volume1en_US
dc.contributor.authorEngel, Larisa
dc.contributor.authorSchneider, Philipp
dc.contributor.authorEbrahimi, Mehrdad
dc.contributor.authorCzermak, Peter
dc.contributor.authoreidpczermaken_US
dc.date.accessioned2010-04-05T21:21:17Z
dc.date.available2010-04-05T21:21:17Z
dc.date.issued2007-11-10
dc.date.published2007en_US
dc.description.abstractA method for immobilization of ß-galactosidase from Kluyveromyces lactis on an adsorptive membrane for the continuous synthesis of galacto-oligosaccharides from lactose was carried out. The immobilization was performed at 4, 10, 15 and 40 °C. Two strongly basic anion exchange membrane with tradename MustangTMQ and SartobindTMQ were investigated. In static experiments, the highest enzyme activity was measured on MustangTMQ membranes at the immobilization temperature of 10 °C. The synthesis of GOS was performed in a Continuous Membrane-Chromatography-Reactor-System (CMCRS) at 40 °C and pH 7.0 using 20 wt% initial lactose concentration. The investigated membranes proved to be a good support for the continuous process at high convective flow rates in the enzyme reactor system. Up to 82 % lactose conversion with 24 % GOS yield was achieved at different fluxes. The corresponding reactor productivity for the production of GOS from lactose in the CCMRS was 98.7 grams GOS per hour and cubic centimeter membrane volume, which significantly exceeds previously reported results.en_US
dc.description.versionArticle (publisher version)
dc.identifier.urihttp://hdl.handle.net/2097/3508
dc.relation.urihttp://doi.org/10.2174/1874256400701010017en_US
dc.rightsThis Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/
dc.subjectImmobilizationen_US
dc.subjectß-galactosidaseen_US
dc.subjectGalacto-oligosaccharidesen_US
dc.subjectAdsorptive membraneen_US
dc.subjectChromatography membraneen_US
dc.subjectMembrane reactoren_US
dc.titleImmobilization of ß-Galactosidase in Adsorptive Membranes for the Continuous Production of Galacto-Oligosaccharides from Lactoseen_US
dc.typeTexten_US

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