Production process for stem cell based therapeutic implants: expansion of the production cell line and cultivation of encapsulated cells
| dc.citation.doi | 10.1007/10_2009_25 | en_US |
| dc.citation.epage | 162 | en_US |
| dc.citation.jtitle | Advances in Biochemical Engineering Biotechnology | en_US |
| dc.citation.spage | 143 | en_US |
| dc.citation.volume | 123 | en_US |
| dc.contributor.author | Weber, C. | |
| dc.contributor.author | Pohl, S. | |
| dc.contributor.author | Poertner, R. | |
| dc.contributor.author | Pino-Grace, P. | |
| dc.contributor.author | Freimark, D. | |
| dc.contributor.author | Wallrapp, C. | |
| dc.contributor.author | Geigle, P. | |
| dc.contributor.author | Czermak, Peter | |
| dc.contributor.authoreid | pczermak | en_US |
| dc.date.accessioned | 2011-04-15T13:56:32Z | |
| dc.date.available | 2011-04-15T13:56:32Z | |
| dc.date.issued | 2011-04-15 | |
| dc.date.published | 2010 | en_US |
| dc.description.abstract | Cell based therapy promises the treatment of many diseases like diabetes mellitus, Parkinson disease or stroke. Microencapsulation of the cells protects them against host-vs-graft reactions and thus enables the usage of allogenic cell lines for the manufacturing of cell therapeutic implants. The production process of such implants consists mainly of the three steps expansion of the cells, encapsulation of the cells, and cultivation of the encapsulated cells in order to increase their vitality and thus quality. This chapter deals with the development of fixed-bed bioreactorbased cultivation procedures used in the first and third step of production. The bioreactor system for the expansion of the stem cell line (hMSC-TERT) is based on non-porous glass spheres, which support cell growth and harvesting with high yield and vitality. The cultivation process for the spherical cell based implants leads to an increase of vitality and additionally enables the application of a medium-based differentiation protocol. | en_US |
| dc.description.version | Article (author version) | |
| dc.identifier.uri | http://hdl.handle.net/2097/8406 | |
| dc.relation.uri | http://doi.org/10.1007/10_2009_25 | en_US |
| dc.rights | This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). | en_US |
| dc.rights.uri | https://rightsstatements.org/page/InC/1.0 | |
| dc.subject | Cell therapy | en_US |
| dc.subject | Encapsulation | en_US |
| dc.subject | Fixed bed bioreactor | en_US |
| dc.subject | Glass carrier | en_US |
| dc.subject | Mesenchymal stem cells | en_US |
| dc.title | Production process for stem cell based therapeutic implants: expansion of the production cell line and cultivation of encapsulated cells | en_US |
| dc.type | Text | en_US |
