Serologic detection of vaccine associate IgG responses in horses using a multiplex magnetic microsphere assay

dc.contributor.authorHaukos, Kaitlin A.
dc.date.accessioned2017-05-04T20:32:18Z
dc.date.available2017-05-04T20:32:18Z
dc.date.graduationmonthAugusten_US
dc.date.issued2017-08-01en_US
dc.date.published2017en_US
dc.description.abstractTo protect horses from disease, equine practitioners typically prescribe a protocol of an initial primary vaccination followed by a booster vaccination 3-4 weeks later. Subsequent boosters are given every 6-12 months depending on the pathogen of concern. Each vaccination incurs an additional cost and increased chance for adverse reactions. Despite wide-spread protocol acceptance, duration of effectiveness of vaccines in protecting horses from disease is not well documented. It was hypothesized that horses vaccinated annually since birth have increased antibody production that remains consistent and sufficient for long-term protection from common diseases. This work resulted in the development of a novel, multiplex-magnetic bead-based indirect immunoassay to screen sera from vaccinated adult horses to measure antibody levels in response to vaccine administration. Antigens tested included West Nile Virus, Eastern Equine Encephalitis, Western Equine Encephalitis, Equine Influenza Virus, Equine Herpes Virus 1 and 4, Tetanus, and 7 different Rabies antigens (3 lab and 4 wild strains). The developed assay was a 7-plex capture antibody, which quantified equine IgG (Immunoglobulin G) that binds viral antigens derived from different rabies virus strains along with pure vaccine samples of the 7 different antigens. A 7-point standard curve was developed to quantify the viral-antigen reactive IgG concentration in vaccinated horse serum. Vaccinated horses increased serum antibody concentration for each antigen post-vaccination with the percent increase ranging between 34.0% for Equine Herpes Virus 4 and 257.3% for Equine Influenza Virus. Use of the novel assay will provide equine veterinarians with an economical method to measure immune activation toward common pathogens of concern. This methodology will provide foundation level information regarding antigen specific IgG concentrations that ultimately may be extrapolated to establish protective levels of immunity resulting in establishment of vaccine protocols.en_US
dc.description.advisorElizabeth G. Davisen_US
dc.description.degreeMaster of Science in Biomedical Sciencesen_US
dc.description.departmentDepartment of Clinical Sciencesen_US
dc.description.levelMastersen_US
dc.description.sponsorshipZoetis LLC KSU-CVM DCS Student Mentor Supporten_US
dc.identifier.urihttp://hdl.handle.net/2097/35556
dc.language.isoen_USen_US
dc.publisherKansas State Universityen
dc.subjectEquineen_US
dc.subjectIgGen_US
dc.subjectVaccine reactionen_US
dc.subjectMicrosphere Assayen_US
dc.subjectSerologyen_US
dc.titleSerologic detection of vaccine associate IgG responses in horses using a multiplex magnetic microsphere assayen_US
dc.typeThesisen_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
KaitilinHaukos2017.pdf
Size:
1.52 MB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.62 KB
Format:
Item-specific license agreed upon to submission
Description: