Lipid profiles in wheat cultivars resistant and susceptible to tan spot and the effect of disease on the profiles

Date

2012-04-13

Journal Title

Journal ISSN

Volume Title

Publisher

Kansas State University

Abstract

The effects of tan spot on lipid profiles in wheat leaves were quantified by mass spectrometry. Inoculation with Pyrenophora tritici-repentis significantly reduced the amount of many lipids, including the major lipids monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), in leaves over time. These two lipids accounted for 89% of the mass spectral signal of detected lipids in wheat leaves. Reductions in amounts of lipids were at much higher rates over time for susceptible cultivars compared with resistant cultivars. Furthermore, data show that cultivars resistant to tan spot have different lipid profiles when compared with susceptible cultivars. Resistant cultivars had more MGDG and DGDG than susceptible ones, even in non-inoculated leaves. Using linear models that were fit to data, non-inoculated cultivars with a rating of 1 (highly resistant to tan spot) were calculated to have 66.1% more MGDG and 52.7% more DGDG signal than cultivars with a rating of 9 (highly susceptible). These latter findings are indirect evidence that the amounts of some lipids in wheat leaves may be determining factors in the resistance response of cultivars to tan spot.

Description

Keywords

Lipid profile, Wheat, Tan spot, Pyrenophora tritici-repentis, Monogalactosyldiacylglycerol (MGDG), Digalactosyldiacylglycerol (DGDG)

Graduation Month

May

Degree

Master of Science

Department

Department of Plant Pathology

Major Professor

William W. Bockus

Date

2012

Type

Thesis

Citation