Restricted nutrient intake does not alter serum-mediated measures of implant response in cell culture

dc.citation.doidoi:10.1186/2049-1891-4-45en_US
dc.citation.issue45en_US
dc.citation.jtitleJournal of Animal Science and Biotechnologyen_US
dc.citation.volume4en_US
dc.contributor.authorReinhardt, Christopher D.
dc.contributor.authorLee, Tiffany L.
dc.contributor.authorThomson, Daniel U.
dc.contributor.authorMamedova, Laman K.
dc.contributor.authorBradford, Barry J.
dc.contributor.authoreidcdr3en_US
dc.contributor.authoreidthomsonen_US
dc.contributor.authoreidmamedovaen_US
dc.contributor.authoreidbbradforen_US
dc.date.accessioned2014-02-24T21:22:33Z
dc.date.available2014-02-24T21:22:33Z
dc.date.issued2014-02-24
dc.date.published2013en_US
dc.description.abstractBackground: During nutritional stress, reduced intake may reduce the efficacy of anabolic implants. This study was conducted to evaluate basic cellular responses to a growth promotant implant at two intake levels. Methods: Sixteen crossbred steers (293 ± 19.3 kg) were used to evaluate the impact of anabolic implants in either an adequate or a restricted nutritional state. Steers were trained to individual Calan gates, and then randomly assigned to 1 of 4 treatments in a 2 × 2 factorial arrangement. Treatments consisted of: presence or absence of an anabolic growth implant (Revalor-XS, 200 mg TBA and 40 mg estradiol; IMPLANT or CONTROL) and a moderate energy, pelleted, starting cattle diet fed at either 2.0 × or 1.0 × maintenance energy (NEM) requirements (HIGH or LOW). Serum (d 0, 14, and 28) was used for application to bovine muscle satellite cells. After treatment with the serum (20% of total media) from the trial cattle, the satellite cells were incubated for 72 h. Protein abundance of myosin heavy chain (MHC), phosphorylated extracellular signal-related kinase (phospho-ERK), and phosphorylated mammalian target of rapamycin (phospho-mTOR) were analyzed to determine the effects of implant, intake, and their interaction (applied via the serum). Results: Intake had no effect on MHC (P = 0.85) but IMPLANT increased (P < 0.01) MHC abundance vs. CONTROL. Implant status, intake status, and the interaction had no effect on the abundance of phospho-ERK (P ≥ 0.23). Implanting increased phospho-mTOR (P < 0.01) but there was no effect (P ≥ 0.51) of intake or intake × implant. Conclusions: The nearly complete lack of interaction between implant and nutritional status indicates that the signaling molecules measured herein respond to implants and nutritional status independently. Furthermore, results suggest that the muscle hypertrophic effects of anabolic implants may not be mediated by circulating IGF-1.en_US
dc.identifier.urihttp://hdl.handle.net/2097/17183
dc.language.isoen_USen_US
dc.relation.urihttp://www.jasbsci.com/content/4/1/45en_US
dc.subjectBeef cattleen_US
dc.subjectImplanten_US
dc.subjectMuscleen_US
dc.subjectMyosin heavy chainen_US
dc.subjectNutrient restrictionen_US
dc.subjectSatellite cellsen_US
dc.titleRestricted nutrient intake does not alter serum-mediated measures of implant response in cell cultureen_US
dc.typeArticle (publisher version)en_US

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