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Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

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dc.citation.doi10.3791/52523
dc.citation.issn1940-087X
dc.citation.issue97
dc.citation.jtitleJove-Journal of Visualized Experiments
dc.citation.spage11
dc.contributor.authorZhang, Xin
dc.contributor.authorMysore, K.
dc.contributor.authorFlannery, E.
dc.contributor.authorMichel, Kristin
dc.contributor.authorSeverson, D. W.
dc.contributor.authorZhu, Kun Y.
dc.contributor.authorDuman-Scheel, M.
dc.contributor.authoreidkmichel
dc.contributor.authoreidkzhu
dc.date.accessioned2016-04-04T22:13:51Z
dc.date.available2016-04-04T22:13:51Z
dc.date.published2015
dc.descriptionCitation: Zhang, X., Mysore, K., Flannery, E., Michel, K., Severson, D. W., Zhu, K. Y., & Duman-Scheel, M. (2015). Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae. Jove-Journal of Visualized Experiments(97), 11. doi:10.3791/52523
dc.descriptionVector mosquitoes inflict more human suffering than any other organism-and kill more than one million people each year. The mosquito genome projects facilitated research in new facets of mosquito biology, including functional genetic studies in the primary African malaria vector Anopheles gambiae and the dengue and yellow fever vector Aedes aegypti. RNA interference-(RNAi-) mediated gene silencing has been used to target genes of interest in both of these disease vector mosquito species. Here, we describe a procedure for preparation of chitosan/interfering RNA nanoparticles that are combined with food and ingested by larvae. This technically straightforward, high-throughput, and relatively inexpensive methodology, which is compatible with long double stranded RNA (dsRNA) or small interfering RNA (siRNA) molecules, has been used for the successful knockdown of a number of different genes in A. gambiae and A. aegypti larvae. Following larval feedings, knockdown, which is verified through qRT-PCR or in situ hybridization, can persist at least through the late pupal stage. This methodology may be applicable to a wide variety of mosquito and other insect species, including agricultural pests, as well as other non-model organisms. In addition to its utility in the research laboratory, in the future, chitosan, an inexpensive, non-toxic and biodegradable polymer, could potentially be utilized in the field.
dc.description.embargoEmbargo 3/25/2017
dc.identifier.urihttp://hdl.handle.net/2097/32244
dc.relation.urihttps://doi.org/10.3791/52523
dc.rightsCopyright © 2015 Journal of Visualized Experiments
dc.rights.urihttp://www.sherpa.ac.uk/romeo/issn/1940-087X/
dc.subjectMolecular Biology
dc.subjectIssue 97
dc.subjectVector Biology
dc.subjectRna Interference
dc.subjectAnopheles
dc.subjectGambiae
dc.titleChitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae
dc.typeArticle

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