Libraries
 

An inactivated vaccine made from a U.S. field isolate of porcine epidemic disease virus is immunogenic in pigs as demonstrated by a dose-titration

Simple item page
dc.citation.doi10.1186/s12917-015-0357-1
dc.citation.issn1746-6148
dc.citation.issue1
dc.citation.jtitleBMC Veterinary Research
dc.citation.volume11
dc.contributor.authorCollin, E. A.
dc.contributor.authorAnbalagan, S.
dc.contributor.authorOkda, F.
dc.contributor.authorBatman, R.
dc.contributor.authorNelson, E.
dc.contributor.authorHause, Benjamin M.
dc.contributor.authoreidbhause
dc.date.accessioned2016-04-04T22:44:58Z
dc.date.available2016-04-04T22:44:58Z
dc.date.issued2015-03-15
dc.date.published2015
dc.descriptionCitation: Collin, E. A., Anbalagan, S., Okda, F., Batman, R., Nelson, E., & Hause, B. M. (2015). An inactivated vaccine made from a U.S. field isolate of porcine epidemic disease virus is immunogenic in pigs as demonstrated by a dose-titration. BMC Veterinary Research, 11(1). doi:10.1186/s12917-015-0357-1
dc.descriptionBackground: Porcine epidemic diarrhea virus (PEDV), a highly pathogenic and transmissible virus in swine, was first detected in the U.S. in May, 2013, and has caused tremendous losses to the swine industry. Due to the difficulty in isolating and growing this virus in cell culture, few vaccine studies using cell culture propagated PEDV have been performed on U.S. strains in pigs. Therefore, the objective of this study was to evaluate the humoral immune response to the selected inactivated PEDV vaccine candidate in a dose-titration manner. Results: PEDV was isolated from a pig with diarrhea and complete genome sequencing found >99% nucleotide identity to other U.S. PEDV. Inactivated adjuvanted monovalent vaccines were administered intramuscularly to five week old pigs in a dose titration experimental design, ranging from 6.0-8.0 log<inf>10</inf> tissue culture infective dose (TCID<inf>50</inf>/mL), to evaluate immunogenicity using a fluorescent foci neutralization assay (FFN), fluorescent microsphere immunoassay (FMIA), and enzyme-linked immunosorbent assay (ELISA) on sera. Pigs vaccinated with 8.0 log<inf>10</inf> TCID<inf>50</inf>/mL inactivated virus showed significantly higher FFN titers as well as FMIA and ELISA values than 6.0 log<inf>10</inf> TCID<inf>50</inf>/mL vaccinates and the negative controls. Conclusions: These results demonstrate the immunogenicity of a PEDV inactivated viral vaccine with a U.S. strain via dose-titration. A future vaccination-challenge study would illustrate the efficacy of an inactivated vaccine and help evaluate protective FFN titers and ELISA and FMIA responses. © Collin et al; licensee BioMed Central.
dc.identifier.urihttp://hdl.handle.net/2097/32282
dc.relation.urihttps://doi.org/10.1186/s12917-015-0357-1
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectDiarrhea
dc.subjectEpidemic
dc.subjectImmunogenicity
dc.subjectInactivated
dc.subjectPedv
dc.subjectPorcine
dc.titleAn inactivated vaccine made from a U.S. field isolate of porcine epidemic disease virus is immunogenic in pigs as demonstrated by a dose-titration
dc.typeArticle

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
art%3A10.1186%2Fs12917-015-0357-1.pdf
Size:
420.62 KB
Format:
Adobe Portable Document Format
Download

Collections

Diagnostic Medicine/Pathobiology Faculty Research and Publications
facebook
twitter
youtube

Center for the Advancement of Digital Scholarship

K-State Libraries

1117 Mid-Campus Drive North, Manhattan, KS 66506

785-532-3514|cads@k-state.edu