Rift Valley fever virus structural and nonstructural proteins: recombinant protein expression and immunoreactivity against antisera from sheep

dc.citation.doi10.1089/vbz.2012.1285en_US
dc.citation.epage629en_US
dc.citation.issue9en_US
dc.citation.jtitleVector-Borne and Zoonotic Diseasesen_US
dc.citation.spage619en_US
dc.citation.volume13en_US
dc.contributor.authorFaburay, Bonto
dc.contributor.authorWilson, William C.
dc.contributor.authorMcVey, D. Scott
dc.contributor.authorDrolet, Barbara S.
dc.contributor.authorWeingartl, Hana
dc.contributor.authorMadden, Daniel
dc.contributor.authorYoung, Alan
dc.contributor.authorMa, Wenjun
dc.contributor.authorRicht, Juergen A.
dc.contributor.authoreidbfaburayen_US
dc.contributor.authoreidwjmaen_US
dc.contributor.authoreidjrichten_US
dc.date.accessioned2013-10-11T18:42:18Z
dc.date.available2013-10-11T18:42:18Z
dc.date.issued2013-10-11
dc.date.issued2013-09-13
dc.date.published2013en_US
dc.description.abstractThe Rift Valley fever virus (RVFV) encodes the structural proteins nucleoprotein (N), aminoterminal glycoprotein (Gn), carboxyterminal glycoprotein (Gc), and L protein, 78-kD, and the nonstructural proteins NSm and NSs. Using the baculovirus system, we expressed the full-length coding sequence of N, NSs, NSm, Gc, and the ectodomain of the coding sequence of the Gn glycoprotein derived from the virulent strain of RVFV ZH548. Western blot analysis using anti-His antibodies and monoclonal antibodies against Gn and N confirmed expression of the recombinant proteins, and in vitro biochemical analysis showed that the two glycoproteins, Gn and Gc, were expressed in glycosylated form. Immunoreactivity profiles of the recombinant proteins in western blot and in indirect enzyme-linked immunosorbent assay against a panel of antisera obtained from vaccinated or wild type (RVFV)-challenged sheep confirmed the results obtained with anti-His antibodies and demonstrated the suitability of the baculo-expressed antigens for diagnostic assays. In addition, these recombinant proteins could be valuable for the development of diagnostic methods that differentiate infected from vaccinated animals (DIVA).en_US
dc.identifier.urihttp://hdl.handle.net/2097/16650
dc.language.isoen_USen_US
dc.relation.urihttp://doi.org/10.1089/vbz.2012.1285en_US
dc.rightsThis is a copy of an article published in Vector-Borne and Zoonotic Diseases © 2013 Mary Ann Liebert, Inc.; Vector-Borne and Zoonotic Diseases is available online at: http://online.liebertpub.com.en_US
dc.subjectRift Valley fever virusen_US
dc.subjectStructural proteinsen_US
dc.subjectNonstructural proteinsen_US
dc.subjectImmunoreactivity profilesen_US
dc.subjectSheepen_US
dc.titleRift Valley fever virus structural and nonstructural proteins: recombinant protein expression and immunoreactivity against antisera from sheepen_US
dc.typeArticle (publisher version)en_US

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