Integrated immunoisolation and protein analysis of circulating exosomes using microfluidic technology

dc.citation.doi10.1039/c4lc00662cen_US
dc.citation.epage3780en_US
dc.citation.issue19en_US
dc.citation.jtitleLab on a Chipen_US
dc.citation.spage3773en_US
dc.citation.volume14en_US
dc.contributor.authorHe, Mei
dc.contributor.authorCrow, Jennifer
dc.contributor.authorRoth, Marc
dc.contributor.authorZeng, Yong
dc.contributor.authorGodwin, Andrew K.
dc.contributor.authoreidmeihen_US
dc.date.accessioned2014-12-02T19:20:51Z
dc.date.available2014-12-02T19:20:51Z
dc.date.issued2014-12-02
dc.date.published2014en_US
dc.description.abstractDeveloping blood-based tests is appealing for non-invasive disease diagnosis, especially when biopsy is difficult, costly, and sometimes not even an option. Tumor-derived exosomes have attracted increasing interest in non-invasive cancer diagnosis and monitoring of treatment response. However, the biology and clinical value of exosomes remains largely unknown due in part to current technical challenges in rapid isolation, molecular classification and comprehensive analysis of exosomes. Here we developed a new microfluidic approach to streamline and expedite the exosome analysis pipeline by integrating specific immunoisolation and targeted protein analysis of circulating exosomes. Compared to the conventional methods, our approach enables selective subpopulation isolation and quantitative detection of surface and intravesicular biomarkers directly from a minimally invasive amount of plasma samples (30 μL) within ~100 min with markedly improved detection sensitivity. Using this device, we demonstrated phenotyping of exosome subpopulations by targeting a panel of common exosomal and tumor-specific markers and multiparameter analyses of intravesicular biomarkers in the selected subpopulation. We were able to assess the total expression and phosphorylation levels of IGF-1R in non-small-cell lung cancer patients by probing plasma exosomes as a non-invasive alternative to conventional tissue biopsy. We foresee that the microfluidic exosome analysis platform will form the basis for critically needed infrastructures for advancing the biology and clinical utilization of exosomes.en_US
dc.identifier.urihttp://hdl.handle.net/2097/18766
dc.language.isoen_USen_US
dc.relation.urihttp://doi.org/10.1039/c4lc00662cen_US
dc.rightsThis article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported license.en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/
dc.subjectImmunoisolationen_US
dc.subjectProtein analysisen_US
dc.subjectCirculating exosomesen_US
dc.subjectMicrofluidic technologyen_US
dc.subjectExosome analysis pipelineen_US
dc.titleIntegrated immunoisolation and protein analysis of circulating exosomes using microfluidic technologyen_US
dc.typeArticle (publisher version)en_US

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