In vitro effects of canine Wharton’s jelly mesenchymal stromal cells and nanoparticles on canine osteosarcoma D17 cell viability.

dc.contributor.authorReeds, Kimberly
dc.date.accessioned2011-08-09T15:09:59Z
dc.date.available2011-08-09T15:09:59Z
dc.date.graduationmonthAugusten_US
dc.date.issued2011-08-09
dc.date.published2011en_US
dc.description.abstractObjectives – To isolate and maintain canine Wharton’s jelly mesenchymal stromal cells (WJMSCs) in culture, to determine the effects of micellar nanoparticles containing doxorubicin (DOX) on WJMSCs and canine osteosarcoma (OSA) D17 cell viability, and to determine the effects of conditioned media from WJMSCs loaded with micellar nanoparticles containing DOX on OSA D17 cell viability. Sample Population – Canine WJMSCs containing various concentrations of DOX micelles and canine OSA D17 cells. Procedures – WJMSCs were isolated from canine umbilical cords. Micellar nanoparticles containing DOX were prepared and added to culture plates containing canine OSA D17 cells to determine micelle effects on cell growth and viability. Conditioned media from culture plates containing canine WJMSCs incubated with various DOX micelle concentrations was added to OSA D17 cells for conditioned media experiments. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to assess OSA D17 cell viability. A trypan blue stain was also utilized to perform cell counts to determine the effect of the DOX micelles on stromal cell growth. Results – WJMSCs were successfully isolated and maintained in culture. Micellar nanoparticles containing DOX decreased OSA D17 cell viability. OSA D17 cell viability was also decreased following incubation with conditioned media from canine WJMSCs loaded with micellar nanoparticles containing DOX. Significant decreases with the conditioned media of canine WJMSCs loaded with 10μM micelles occurred at 48 hours (p < 0.005) and at 72 and 96 hours (p < 0.0001). Significant decreases were also observed with the 1 μM DOX micelles at 72 hours (p < 0.005) and 96 hours (p < 0.0001). WJMSC numbers decreased in a dose dependent manner following incubation with DOX micelles. Changes in WJMSC number was not caused by increased cell death as all variables produced similar percentages of dead cells. Conclusions – Canine WJMSCs were successfully isolated and maintained in culture. Stromal cells containing DOX micellar nanoparticles induced OSA D17 cell cytotoxicity while inducing an anti-proliferative, rather than cytotoxic effect, on the WJMSC. These data support future in vivo experiments utilizing canine WJMSCs and micellar nanoparticles.en_US
dc.description.advisorMary Lynn Higginbothamen_US
dc.description.degreeMaster of Scienceen_US
dc.description.departmentDepartment of Clinical Sciencesen_US
dc.description.levelMastersen_US
dc.description.sponsorshipNational Institutes of Health 1R21CA135599 and Johnson Cancer Center, KSUen_US
dc.identifier.urihttp://hdl.handle.net/2097/11990
dc.language.isoen_USen_US
dc.publisherKansas State Universityen
dc.subjectOsteosarcomaen_US
dc.subjectDoxorubicinen_US
dc.subjectNanoparticlesen_US
dc.subjectWharton's jelly mesenchymal stromal cellsen_US
dc.subjectPolymeric micellesen_US
dc.subject.umiNanoscience (0565)en_US
dc.subject.umiOncology (0992)en_US
dc.subject.umiVeterinary Medicine (0778)en_US
dc.titleIn vitro effects of canine Wharton’s jelly mesenchymal stromal cells and nanoparticles on canine osteosarcoma D17 cell viability.en_US
dc.typeThesisen_US

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