Impact of dietary nitrate supplementation via beetroot juice on exercising muscle vascular control in rats

dc.contributor.authorFerguson, Scott Kohman
dc.date.accessioned2012-11-27T14:31:34Z
dc.date.available2012-11-27T14:31:34Z
dc.date.graduationmonthDecemberen_US
dc.date.issued2012-11-27
dc.date.published2012en_US
dc.description.abstractIntroduction: Dietary nitrate(NO[subscript]3[superscript]-) supplementation, via its reduction to nitrite (NO [subscript] 2 [superscript]-) and subsequent conversion to nitric oxide (NO) and other reactive nitrogen intermediates, reduces blood pressure and the O[subscript]2 cost of submaximal exercise in humans. Despite these observations, the effects of dietary NO [subscript]3 [superscript]- supplementation on skeletal muscle vascular control during locomotory exercise remain unknown. We tested the hypotheses that dietary NO [subscript]3 [superscript]- supplementation via beetroot juice (BR) would reduce mean arterial pressure (MAP) and increase hindlimb muscle blood flow in the exercising rat. Methods: Male Sprague-Dawley rats (3-6 months) were administered either NO [subscript]3 [superscript]- (via beetroot juice; 1 mmol · kg[superscript]-[superscript]1 · day[superscript]-[superscript]1, BR n=8) or untreated (control, n=11) tap water for 5 days. MAP and hindlimb skeletal muscle blood flow and vascular conductance (radiolabeled microsphere infusions) were measured during submaximal treadmill running (20 m · min[superscript]-[superscript]1, 5% grade). Results: BR resulted in significantly lower exercising MAP (control: 137 ± 3, BR: 127 ± 4 mmHg, P<0.05) and blood [lactate] (control: 2.6 ± 0.3, BR: 1.9 ± 0.2 mM, P<0.05) compared to control. Total exercising hindlimb skeletal muscle blood flow (control: 108 ± 8, BR: 150 ± 11 ml · min[superscript]-[superscript]1 · 100 g[superscript]-[superscript]1, P<0.05) and vascular conductance (control: 0.78 ± 0.05, BR: 1.16 ± 0.10 ml · min[superscript]-[superscript]1 · 100 g[superscript]-[superscript]1 · mmHg[superscript]-[superscript]1, P<0.05) were greater in rats that received beetroot juice compared to control. The relative differences in blood flow and vascular conductance for the 28 individual hindlimb muscles and muscle parts correlated positively with their percent type IIb + d/x muscle fibers (blood flow: r=0.74, vascular conductance: r=0.71, P<0.01 for both). Conclusion: These data support the hypothesis that NO [subscript]3 [superscript]- supplementation improves vascular control and elevates skeletal muscle O [subscript]2 delivery during exercise predominantly in fast-twitch type II muscles, and provide a potential mechanism by which NO [subscript]3 [superscript]- supplementation improves metabolic control.en_US
dc.description.advisorDavid C. Pooleen_US
dc.description.degreeMaster of Scienceen_US
dc.description.departmentDepartment of Kinesiologyen_US
dc.description.levelMastersen_US
dc.identifier.urihttp://hdl.handle.net/2097/15048
dc.language.isoen_USen_US
dc.publisherKansas State Universityen
dc.subjectNitric Oxideen_US
dc.subjectBlood flowen_US
dc.subjectDietaryen_US
dc.subjectNitrateen_US
dc.subject.umiPhysiology (0719)en_US
dc.titleImpact of dietary nitrate supplementation via beetroot juice on exercising muscle vascular control in ratsen_US
dc.typeThesisen_US

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