Host identity impacts rhizosphere fungal communities associated with three alpine plant species
| dc.citation.doi | doi:10.1007/s00248-011-9968-7 | en_US |
| dc.citation.epage | 693 | en_US |
| dc.citation.issue | 3 | en_US |
| dc.citation.jtitle | Microbial Ecology | en_US |
| dc.citation.spage | 682 | en_US |
| dc.citation.volume | 63 | en_US |
| dc.contributor.author | Becklin, Katie M. | |
| dc.contributor.author | Hertweck, Kate L. | |
| dc.contributor.author | Jumpponen, Ari M. | |
| dc.contributor.authoreid | ari | en_US |
| dc.date.accessioned | 2012-07-05T20:01:02Z | |
| dc.date.available | 2012-07-05T20:01:02Z | |
| dc.date.issued | 2012-07-05 | |
| dc.date.published | 2012 | en_US |
| dc.description.abstract | Fungal diversity and composition are still relatively unknown in many ecosystems; however, host identity and environmental conditions are hypothesized to influence fungal community assembly. To test these hypotheses we characterized the richness, diversity, and composition of rhizosphere fungi colonizing three alpine plant species, Taraxacum ceratophorum, Taraxacum officinale, and Polemonium viscosum. Roots were collected from open meadow and willow understory habitats at treeline on Pennsylvania Mountain, Colorado, USA. Fungal small subunit ribosomal DNA was sequenced using fungal-specific primers, sample-specific DNA tags, and 454 pyrosequencing. We classified operational taxonomic units (OTUs) as arbuscular mycorrhizal (AMF) or non-arbuscular mycorrhizal (non-AMF) fungi, then tested whether habitat or host identity influenced these fungal communities. Approximately 14% of the sequences represented AMF taxa (44 OTUs) with the majority belonging to Glomus group A and B. NONAMF sequences represented 186 OTUs belonging to Ascomycota (58%), Basidiomycota (26%), Zygomycota (14%), and Chytridiomycota (2%) phyla. Total AMF and non-AMF richness were similar between habitats, but varied among host species. AMF richness and diversity per root sample also varied among host species and were highest in T. ceratophorum compared to T. officinale and P. viscosum. In contrast, non-AMF richness and diversity per root sample were similar among host species except in the willow understory where diversity was reduced in T. officinale. Fungal community composition was influenced by host identity, but not habitat. Specifically, T. officinale hosted a different AMF community than T. ceratophorum and P. viscosum, while P. viscosum hosted a different non-AMF community than T. ceratophorum and T. officinale. Our results suggest that host identity has a stronger effect on rhizosphere fungi than habitat. Furthermore, although host identity influenced both AMF and non-AMF this effect was stronger for the mutualistic AMF community. | en_US |
| dc.identifier.uri | http://hdl.handle.net/2097/13983 | |
| dc.relation.uri | http://www.springerlink.com/content/10304t3740514v25/ | en_US |
| dc.rights | The final publication is available at www.springerlink.com | en_US |
| dc.subject | Host identity | en_US |
| dc.subject | Rhizosphere fungal communities | en_US |
| dc.title | Host identity impacts rhizosphere fungal communities associated with three alpine plant species | en_US |
| dc.type | Article (author version) | en_US |
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- Supplementary Table 1: GenBank accession number, top BLASTN match, and phylogenetic assignment for each 454 operational taxonomic unit (OTU). OTUs were designated based on 97% sequence similarity.
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- Supplementary Table 2: Number of sequences, number of operational taxonomic units (OTUs), and relative abundance of each OTU per root sample collected from T. ceratophorum, T. officinale, and P. viscosum plants in open meadow and willow understory habitats. OTUs were designated based on 97% sequence similarity.
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- Supplementary Figure 1: Mean (± SE) AMF richness, diversity, and evenness per root sample based on OTUs designated at 90, 95, 97, and 99% sequence similarity. (a,d) OTU richness by habitat (a) and host species (d); (b,e) Shannon’s diversity index by habitat (b) and host species (e); (c,f) OTU evenness by habitat (c) and host species (f). In panels a–c open and solid circles represent samples from the open meadow and willow understory, respectively. In panels d–f symbols represent samples from T. ceratophorum (circles), T. officinale (squares), and P. viscosum (triangles).
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- Supplementary Figure 2: Mean (± SE) non-AMF richness, diversity, and evenness per root sample based on OTUs designated at 90%, 95%, 97%, and 99% sequence similarity. (a) OTU richness by habitat and host species; (b) Shannon’s diversity index by habitat and host species; (c) OTU evenness by habitat and host species. Symbols represent samples from T. ceratophorum (circles), T. officinale (squares), and P. viscosum (triangles).
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