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Precisely mapping a major gene conferring resistance to Hessian fly in bread wheat using genotyping-by-sequencing

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dc.citationLi, G. Q., Wang, Y., Chen, M. S., Edae, E., Poland, J., Akhunov, E., . . . Yan, L. L. (2015). Precisely mapping a major gene conferring resistance to Hessian fly in bread wheat using genotyping-by-sequencing. Bmc Genomics, 16, 10. doi:10.1186/s12864-015-1297-7
dc.citation.doi10.1186/s12864-015-1297-7
dc.citation.issn1471-2164
dc.citation.jtitleBmc Genomics
dc.citation.spage10
dc.citation.volume16
dc.contributor.authorLi, G. Q.
dc.contributor.authorWang, Y.
dc.contributor.authorChen, M. S.
dc.contributor.authorEdae, E.
dc.contributor.authorPoland, Jesse A.
dc.contributor.authorAkhunov, Eduard
dc.contributor.authorChao, S. M.
dc.contributor.authorBai, G. H.
dc.contributor.authorCarver, B. F.
dc.contributor.authorYan, L. L.
dc.contributor.authoreidjpoland
dc.contributor.authoreideakhunov
dc.date.accessioned2016-04-06T15:14:25Z
dc.date.available2016-04-06T15:14:25Z
dc.date.issued2015-02-21
dc.date.published2015
dc.descriptionCitation: Li, G. Q., Wang, Y., Chen, M. S., Edae, E., Poland, J., Akhunov, E., . . . Yan, L. L. (2015). Precisely mapping a major gene conferring resistance to Hessian fly in bread wheat using genotyping-by-sequencing. Bmc Genomics, 16, 10. doi:10.1186/s12864-015-1297-7
dc.descriptionBackground: One of the reasons hard red winter wheat cultivar 'Duster' (PI 644016) is widely grown in the southern Great Plains is that it confers a consistently high level of resistance to biotype GP of Hessian fly (Hf). However, little is known about the genetic mechanism underlying Hf resistance in Duster. This study aimed to unravel complex structures of the Hf region on chromosome 1AS in wheat by using genotyping-by-sequencing (GBS) markers and single nucleotide polymorphism (SNP) markers. Results: Doubled haploid (DH) lines generated from a cross between two winter wheat cultivars, 'Duster' and 'Billings', were used to identify genes in Duster responsible for effective and consistent resistance to Hf. Segregation in reaction of the 282 DH lines to Hf biotype GP fit a one-gene model. The DH population was genotyped using 2,358 markers developed using the GBS approach. A major QTL, explaining 88% of the total phenotypic variation, was mapped to a chromosome region that spanned 178 cM and contained 205 GBSmarkers plus 1 SSR marker and 1 gene marker, with 0.86 cM per marker in genetic distance. The analyses of GBS marker sequences and further mapping of SSR and gene markers enabled location of the QTL-containing linkage group on the short arm of chromosome 1A. Comparative mapping of the common markers for the gene for QHf. osu-1A(d) in Duster and the Hf-resistance gene for QHf.osu-1A(74) in cultivar '2174' showed that the two Hf resistance genes are located on the same chromosome arm 1AS, only 11.2 cM apart in genetic distance. The gene at QHf. osu-1A(d) in Duster has been delimited within a 2.7 cM region. Conclusion: Two distinct resistance genes exist on the short arm of chromosome 1A as found in the two hard red winter cultivars, 2174 and Duster. Whereas the Hf resistance gene in 2174 is likely allelic to one or more of the previously mapped resistance genes (H9, H10, H11, H16, or H17) in wheat, the gene in Duster is novel and confers a more consistent phenotype than 2174 in response to biotype GP infestation in controlled-environment assays.
dc.description.versionArticle: Version of Record
dc.identifier.urihttp://hdl.handle.net/2097/32465
dc.relation.urihttps://doi.org/10.1186/s12864-015-1297-7
dc.rightsAttribution 4.0 International (CC BY 4.0). This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttps://www.biomedcentral.com/getpublished/copyright-and-license
dc.subjectHessian Fly Resistance
dc.subjectGenotyping-By-Sequencing (Gbs)
dc.subjectInsect
dc.subjectResistance Pathway
dc.subjectQuantitative Trait Loci (Qtl)
dc.subjectWheat
dc.titlePrecisely mapping a major gene conferring resistance to Hessian fly in bread wheat using genotyping-by-sequencing
dc.typeText

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