Impact of treatment strategies on cephalosporin and tetracycline resistance gene quantities in the bovine fecal metagenome
dc.citation.doi | 10.1038/srep05100 | en_US |
dc.citation.epage | 10 | en_US |
dc.citation.issue | Article number: 5100 | en_US |
dc.citation.jtitle | Scientific Reports | en_US |
dc.citation.spage | 1 | en_US |
dc.citation.volume | 4 | en_US |
dc.contributor.author | Kanwar, Neena | |
dc.contributor.author | Scott, Harvey Morgan | |
dc.contributor.author | Norby, Bo | |
dc.contributor.author | Loneragan, Guy H. | |
dc.contributor.author | Vinasco-Torres, Javier | |
dc.contributor.author | Cottell, Jennifer L. | |
dc.contributor.author | Chalmers, Gabhan | |
dc.contributor.author | Chengappa, Muckatira M. | |
dc.contributor.author | Bai, Jianfa | |
dc.contributor.author | Boerlin, Patrick | |
dc.contributor.authoreid | hmscott | en_US |
dc.contributor.authoreid | jvinasco | en_US |
dc.contributor.authoreid | chengapa | en_US |
dc.contributor.authoreid | jbai | en_US |
dc.date.accessioned | 2014-07-23T19:33:50Z | |
dc.date.available | 2014-07-23T19:33:50Z | |
dc.date.issued | 2014-05-29 | |
dc.date.published | 2014 | en_US |
dc.description.abstract | The study objective was to determine the effects of two treatment regimens on quantities of ceftiofur and tetracycline resistance genes in feedlot cattle. The two regimens were ceftiofur crystalline-free acid (CCFA) administered to either one or all steers within a pen and subsequent feeding/not feeding of therapeutic doses of chlortetracycline. A 26-day randomized controlled field trial was conducted on 176 steers. Real-time PCR was used to quantify bla[subscript CMY-2], bla[subscript CTX-M], tet(A), tet(B), and 16S rRNA gene copies/gram of feces from community DNA. A significant increase in ceftiofur resistance and a decrease in tetracycline resistance elements were observed among the treatment groups in which all steers received CCFA treatment, expressed as gene copies/gram of feces. Subsequent chlortetracycline administration led to rapid expansion of both ceftiofur and tetracycline resistance gene copies/gram of feces. Our data suggest that chlortetracycline is contraindicated when attempting to avoid expansion of resistance to critically important third-generation cephalosporins. | en_US |
dc.identifier.uri | http://hdl.handle.net/2097/18130 | |
dc.language.iso | en_US | en_US |
dc.relation.uri | http://www.doi.org/10.1038/srep05100 | en_US |
dc.subject | Chlortetracycline | en_US |
dc.subject | Ceftiofur resistance | en_US |
dc.subject | Tetracycline resistance | en_US |
dc.title | Impact of treatment strategies on cephalosporin and tetracycline resistance gene quantities in the bovine fecal metagenome | en_US |
dc.type | Article (publisher version) | en_US |
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