This report documents measurement of the 3D geometrical properties associated with prestressed concrete railroad ties that have been subject to long-term in-track loading. A commercially-available 3D laser-based optical scanning system was used to scan the surface of sampled in-service crossties to a spatial resolution of about 1-mm, resulting in a 3D solid body CAD model of each scanned tie. A high-speed algorithm was developed to process the detailed cross-section geometrical parameters to an axial resolution of 0.5 inches, achieving an overall processing time of only a few minutes. The agreement between the ideal CAD model cross-section parameters and the measured (scanned) parameters was found to be excellent. Measured crosstie cross-sectional parameters used in support of on-going FRA project work included cross-sectional area, area moment of inertia, neutral axis position, eccentricity, and shape factor.