Phenotypic, genetic, and transcriptomic decoupling of thermal hardiness across metamorphosis in Drosophila melanogaster

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dc.contributor.author Freda, Philip John
dc.date.accessioned 2018-11-09T16:36:41Z
dc.date.available 2018-11-09T16:36:41Z
dc.date.issued 2018-12-01
dc.identifier.uri http://hdl.handle.net/2097/39249
dc.description.abstract Complex life cycles (CLCs), developmental programs in which life-history stages are distinct in morphology, behavior, and physiology, are common throughout the biosphere. However, it is still unclear why and how CLCs evolve. The adaptive decoupling hypothesis (ADH) postulates that CLCs evolve to decouple the developmental processes that underlie traits across ontogeny to allow for independent, stage-specific responses to selection. This ultimately could lead to alternate life-history stages adapting to unique environments, thus optimizing fitness across development. However, few empirical tests of the ADH are available. Detecting genetic and transcriptomic decoupling of thermal hardiness using robust techniques in a model system, D. melanogaster, was the goal of this dissertation. Furthermore, this work illustrates that different life-history stages have the potential to become adapted to unique ecological niches. I performed three primary studies to test the ADH: 1.) estimation of the genetic correlation for cold hardiness between larvae and adults using isogenic lines of D. melanogaster to determine if unique genetic architectures underlie variation in cold stress response using standard quantitative genetic and Genome-Wide Association (GWA) methods, 2.) testing whether developmental acclimation is genetically correlated across stages, and whether acclimation alters cross-stage correlations in cold hardiness, and 3.) analysis of the transcriptional responses of both larvae and adults to extreme cold to determine if stage-specific stress response mechanisms exist across development. en_US
dc.description.sponsorship The National Science Foundation Kansas State Agricultural Research and Extension en_US
dc.language.iso en_US en_US
dc.subject Drosophila en_US
dc.subject metamorphosis en_US
dc.subject decoupling en_US
dc.subject genetic en_US
dc.subject transcriptomic en_US
dc.subject phenotypic en_US
dc.title Phenotypic, genetic, and transcriptomic decoupling of thermal hardiness across metamorphosis in Drosophila melanogaster en_US
dc.type Dissertation en_US
dc.description.degree Doctor of Philosophy en_US
dc.description.level Doctoral en_US
dc.description.department Department of Entomology en_US
dc.description.advisor Yoonseong Park en_US
dc.date.published 2018 en_US
dc.date.graduationmonth December en_US


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