Targeted Amplicon Sequencing for Single-Nucleotide-Polymorphism Genotyping of Attaching and Effacing Escherichia coli O26:H11 Cattle Strains via a High-Throughput Library Preparation Technique

K-REx Repository

Show simple item record

dc.contributor.author Ison, Sarah A.
dc.contributor.author Delannoy, Sabine
dc.contributor.author Bugarel, Marie
dc.contributor.author Nagaraja, Tiruvoor G.
dc.contributor.author Renter, David G.
dc.contributor.author Bakker, Henk C. den
dc.contributor.author Nightingale, Kendra K.
dc.contributor.author Fach, Patrick
dc.contributor.author Loneragan, Guy H.
dc.date.accessioned 2018-07-25T21:44:05Z
dc.date.available 2018-07-25T21:44:05Z
dc.date.issued 2016-01-15
dc.identifier.uri http://hdl.handle.net/2097/39061
dc.description.abstract Enterohemorrhagic Escherichia coli (EHEC) O26:H11, a serotype within Shiga toxin-producing E. coli (STEC) that causes severe human disease, has been considered to have evolved from attaching and effacing E. coli (AEEC) O26:H11 through the acquisition of a Shiga toxin-encoding gene. Targeted amplicon sequencing using next-generation sequencing technology of 48 phylogenetically informative single-nucleotide polymorphisms (SNPs) and three SNPs differentiating Shiga toxin-positive (stx-positive) strains from Shiga toxin-negative (stx-negative) strains were used to infer the phylogenetic relationships of 178 E. coli O26:H11 strains (6 stx-positive strains and 172 stx-negative AEEC strains) from cattle feces to 7 publically available genomes of human clinical strains. The AEEC cattle strains displayed synonymous SNP genotypes with stx 2-positive sequence type 29 (ST29) human O26:H11 strains, while stx 1 ST21 human and cattle strains clustered separately, demonstrating the close phylogenetic relatedness of these Shiga toxin-negative AEEC cattle strains and human clinical strains. With the exception of seven stx-negative strains, five of which contained espK, three stx-related SNPs differentiated the STEC strains from non-STEC strains, supporting the hypothesis that these AEEC cattle strains could serve as a potential reservoir for new or existing pathogenic human strains. Our results support the idea that targeted amplicon sequencing for SNP genotyping expedites strain identification and genetic characterization of E. coli O26:H11, which is important for food safety and public health.
dc.relation.uri https://doi.org/10.1128/AEM.03182-15
dc.rights © 2016, American Society for Microbiology. All Rights Reserved. This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.rights.uri http://journals.asm.org/site/misc/ASM_Author_Statement.xhtml
dc.title Targeted Amplicon Sequencing for Single-Nucleotide-Polymorphism Genotyping of Attaching and Effacing Escherichia coli O26:H11 Cattle Strains via a High-Throughput Library Preparation Technique
dc.type Text
dc.date.published 2016
dc.citation.doi 10.1128/AEM.03182-15
dc.citation.epage 649
dc.citation.issn 0099-2240
dc.citation.issue 2
dc.citation.jtitle Applied and Environmental Microbiology
dc.citation.spage 640
dc.citation.volume 82
dc.description.version Article: Version of Record


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search K-REx


Browse

My Account

Statistics








Center for the

Advancement of Digital

Scholarship

cads@k-state.edu