Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae

Xia, P. P.; Wang, Y. T.; Zhu, C. R.; Zou, Y. J.; Yang, Y.; Liu, W.; Hardwidge, Philip R.; Zhu, G. Q.

doi:10.1186/s13567-016-0313-5

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dc.contributor.author	Xia, P. P.
dc.contributor.author	Wang, Y. T.
dc.contributor.author	Zhu, C. R.
dc.contributor.author	Zou, Y. J.
dc.contributor.author	Yang, Y.
dc.contributor.author	Liu, W.
dc.contributor.author	Hardwidge, Philip R.
dc.contributor.author	Zhu, G. Q.
dc.date.accessioned	2016-09-20T17:34:06Z
dc.date.available	2016-09-20T17:34:06Z
dc.date.issued	2016-02-09
dc.identifier.uri	http://hdl.handle.net/2097/34068
dc.description	Citation: Xia, P. P., Wang, Y. T., Zhu, C. R., Zou, Y. J., Yang, Y., Liu, W., . . . Zhu, G. Q. (2016). Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae. Veterinary Research, 47, 7. doi:10.1186/s13567-016-0313-5
dc.description.abstract	F4(+) enterotoxigenic Escherichia coli (ETEC) strains cause diarrheal disease in neonatal and post-weaned piglets. Several different host receptors for F4 fimbriae have been described, with porcine aminopeptidase N (APN) reported most recently. The FaeG subunit is essential for the binding of the three F4 variants to host cells. Here we show in both yeast two-hybrid and pulldown assays that APN binds directly to FaeG, the major subunit of F4 fimbriae, from three serotypes of F4(+) ETEC. Modulating APN gene expression in IPEC-J2 cells affected ETEC adherence. Antibodies raised against APN or F4 fimbriae both reduced ETEC adherence. Thus, APN mediates the attachment of F4(+) E. coli to intestinal epithelial cells.
dc.relation.uri	https://doi.org/10.1186/s13567-016-0313-5
dc.rights	Attribution 4.0 International (CC BY 4.0)
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	F4 Fimbriae
dc.subject	Brush-Borders
dc.subject	In-Vitro
dc.subject	Adhesin Variants
dc.subject	Receptor-Binding
dc.subject	Epithelial-Cell
dc.title	Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae
dc.type	Article
dc.date.published	2016
dc.citation.doi	10.1186/s13567-016-0313-5
dc.citation.issn	2151-0032
dc.citation.jtitle	Veterinary Research
dc.citation.spage	7
dc.citation.volume	47
dc.contributor.authoreid	philiphardwidge