dc.contributor.author |
Xia, P. P. |
|
dc.contributor.author |
Wang, Y. T. |
|
dc.contributor.author |
Zhu, C. R. |
|
dc.contributor.author |
Zou, Y. J. |
|
dc.contributor.author |
Yang, Y. |
|
dc.contributor.author |
Liu, W. |
|
dc.contributor.author |
Hardwidge, Philip R. |
|
dc.contributor.author |
Zhu, G. Q. |
|
dc.date.accessioned |
2016-09-20T17:34:06Z |
|
dc.date.available |
2016-09-20T17:34:06Z |
|
dc.date.issued |
2016-02-09 |
|
dc.identifier.uri |
http://hdl.handle.net/2097/34068 |
|
dc.description |
Citation: Xia, P. P., Wang, Y. T., Zhu, C. R., Zou, Y. J., Yang, Y., Liu, W., . . . Zhu, G. Q. (2016). Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae. Veterinary Research, 47, 7. doi:10.1186/s13567-016-0313-5 |
|
dc.description.abstract |
F4(+) enterotoxigenic Escherichia coli (ETEC) strains cause diarrheal disease in neonatal and post-weaned piglets. Several different host receptors for F4 fimbriae have been described, with porcine aminopeptidase N (APN) reported most recently. The FaeG subunit is essential for the binding of the three F4 variants to host cells. Here we show in both yeast two-hybrid and pulldown assays that APN binds directly to FaeG, the major subunit of F4 fimbriae, from three serotypes of F4(+) ETEC. Modulating APN gene expression in IPEC-J2 cells affected ETEC adherence. Antibodies raised against APN or F4 fimbriae both reduced ETEC adherence. Thus, APN mediates the attachment of F4(+) E. coli to intestinal epithelial cells. |
|
dc.relation.uri |
https://doi.org/10.1186/s13567-016-0313-5 |
|
dc.rights |
Attribution 4.0 International (CC BY 4.0) |
|
dc.rights.uri |
https://creativecommons.org/licenses/by/4.0/ |
|
dc.subject |
F4 Fimbriae |
|
dc.subject |
Brush-Borders |
|
dc.subject |
In-Vitro |
|
dc.subject |
Adhesin Variants |
|
dc.subject |
Receptor-Binding |
|
dc.subject |
Epithelial-Cell |
|
dc.title |
Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae |
|
dc.type |
Article |
|
dc.date.published |
2016 |
|
dc.citation.doi |
10.1186/s13567-016-0313-5 |
|
dc.citation.issn |
2151-0032 |
|
dc.citation.jtitle |
Veterinary Research |
|
dc.citation.spage |
7 |
|
dc.citation.volume |
47 |
|
dc.contributor.authoreid |
philiphardwidge |
|