Selection of reference genes for expression analysis in Diuraphis noxia (Hemiptera: Aphididae) fed on resistant and susceptible wheat plants

K-REx Repository

Show simple item record

dc.contributor.author Sinha, Deepak K.
dc.contributor.author Smith, C. Michael
dc.date.accessioned 2014-07-23T19:41:25Z
dc.date.available 2014-07-23T19:41:25Z
dc.date.issued 2014-07-23
dc.identifier.uri http://hdl.handle.net/2097/18131
dc.description Citation: Sinha, D.K. & Smith, C.M. Selection of Reference Genes for Expression Analysis in Diuraphis noxia (Hemiptera: Aphididae) Fed on Resistant and Susceptible Wheat Plants. Sci. Rep. 4, 5059; DOI:10.1038/srep05059 (2014).
dc.description.abstract The Russian wheat aphid (RWA), Diuraphis noxia Kurdjumov, is a major global pest of wheat and barley production that causes enormous economic damage. Few studies have been conducted to explore and decipher the molecular basis of RWA strategies to evade plant defense mechanisms. Gene expression studies of RWA in response to wheat genotypes carrying different RWA resistance genes have been initiated in our group; however, a secure and accurate understanding of RWA gene expression is dependent on identification of suitable reference genes. This study analyzed expression profiles of five potential reference genes selected and sequenced during RNA sequencing experiments. The expression of genes coding for actin and ribosomal protein L27 was comparatively less variable in RWA fed on different wheat hosts. Results of geNorm, NormFinder, and BestKeeper expression analyses support the use of actin and ribosomal protein L27 in RT-qPCR studies of RWA gene expression in studies involving RWA-wheat interactions. en_US
dc.language.iso en_US en_US
dc.relation.uri https://doi.org/srep05059 en_US
dc.rights This work is licensed under a Creative Commons Attribution-NonCommercialNoDerivs 3.0 Unported License. The images in this article are included in the article’s Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permissionfrom the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject Russian wheat aphid en_US
dc.subject Diuraphis noxia Kurdjumov en_US
dc.subject Gene expression en_US
dc.subject Wheat en_US
dc.title Selection of reference genes for expression analysis in Diuraphis noxia (Hemiptera: Aphididae) fed on resistant and susceptible wheat plants en_US
dc.type Text en_US
dc.date.published 2014 en_US
dc.citation.doi 10.1038/srep05059 en_US
dc.citation.epage 6 en_US
dc.citation.issn 2045-2322
dc.citation.issue Article number: 5059 en_US
dc.citation.jtitle Scientific Reports en_US
dc.citation.spage 1 en_US
dc.citation.volume 4 en_US
dc.citation Sinha, D.K. & Smith, C.M. Selection of Reference Genes for Expression Analysis in Diuraphis noxia (Hemiptera: Aphididae) Fed on Resistant and Susceptible Wheat Plants. Sci. Rep. 4, 5059; DOI:10.1038/srep05059 (2014).
dc.contributor.authoreid dksinha en_US
dc.contributor.authoreid cmsmith en_US
dc.description.version Article: Publisher version


Files in this item

This item appears in the following Collection(s)

Show simple item record

This work is licensed under a Creative Commons Attribution-NonCommercialNoDerivs 3.0 Unported License. The images in this article are included in the article’s Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permissionfrom the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ Except where otherwise noted, the use of this item is bound by the following: This work is licensed under a Creative Commons Attribution-NonCommercialNoDerivs 3.0 Unported License. The images in this article are included in the article’s Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permissionfrom the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

Search K-REx


Browse

My Account

Statistics








Center for the

Advancement of Digital

Scholarship

cads@k-state.edu