Influence of process conditions on measles virus stability

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Show simple item record Weiss, K. Salzig, D. Röder, Y. Gerstenberger, J. Mühlebach, M. D. Cichutek, K. Pörtner, R. Czermak, Peter 2013-08-13T20:32:09Z 2013-08-13T20:32:09Z 2013-08-13
dc.description.abstract Recombinant measles viruses are currently tested in clinical trials as oncolytic agent to be applied in cancer therapy. Contrary to their use as vaccine where 10³ infectious virus particles per dose are needed, for cancer therapy 10[superscript 9] virus particles should be provided per dose. This leads to other challenges for the production process when compared to vaccine production. This study presents measles virus stability with regard to conditions during production and storage of the virus. Relevant process parameters such as temperature (4-37°C), pH (pH 4-11), conductivity (1.5 to 137.5 mS cmˉ¹) and oxygen partial pressure were analyzed. The infectivity of measles virus particles decreased highly at 37 and 32°C, while at 22 and 4°C it remained stable for several hours or even days, respectively. The thermal inactivation reactions followed first order kinetics and the thermodynamic parameters enthalpy and entropy were estimated. Towards changes in pH measles virus particles were very sensitive, while no inactivation could be observed with varying conductivity. Measles virus incubation at an oxygen partial pressure of 100% did not lead to any loss of infectivity. The results show which parameters should be considered and controlled strongly in the production process to further raise measles virus yields for the high amount needed in cancer therapy approaches. en_US
dc.language.iso en_US en_US
dc.relation.uri en_US
dc.subject Measles virus en_US
dc.subject Virus stability en_US
dc.subject Virus inactivation en_US
dc.subject Oncolytic agent en_US
dc.title Influence of process conditions on measles virus stability en_US
dc.type Article (publisher version) en_US 2013 en_US
dc.citation.doi doi:10.3844/ajbbsp.2013.243.254 en_US
dc.citation.epage 254 en_US
dc.citation.issue 3 en_US
dc.citation.jtitle American Journal of Biochemistry and Biotechnology en_US
dc.citation.spage 243 en_US
dc.citation.volume 9 en_US
dc.contributor.authoreid pczermak en_US

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