Rat cardiomyocytes express a classical epithelial beta-defensin

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dc.contributor.author Linde, Annika
dc.contributor.author Lushington, Gerald H.
dc.contributor.author Blecha, Frank
dc.contributor.author Melgarejo, Tonatiuh
dc.date.accessioned 2013-05-29T20:31:08Z
dc.date.available 2013-05-29T20:31:08Z
dc.date.issued 2013-05-29
dc.identifier.uri http://hdl.handle.net/2097/15861
dc.description.abstract Beta-defensins (BDs) are classical epithelial antimicrobial peptides of immediate importance in innate host defense. Since recent studies have suggested that certain BDs are also expressed in non-traditional tissues, including whole heart homogenate and because effector molecules of innate immunity and inflammation can influence the development of certain cardiovascular disease processes, we hypothesized that BDs are produced by cardiomyocytes as a local measure of cardioprotection against danger signals. Here we report that at least one rat beta-defensin, rBD1, is expressed constitutively in cardiomyocytes specifically isolated using position-ablation-laser-microdissection (P.A.L.M. Microlaser Technologies). RT-PCR analysis showed expression of a single 318 bp transcript in adult rat heart (laser-excised cardiomyocytes) and H9c2 cells (neonatal rat heart myoblasts). Moreover, the full length cDNA of rBD1 was established and translated into a putative peptide with 69 amino acid residues. The predicted amino acid sequence of the adult rat cardiac BD-1 peptide displayed 99% identity with the previously reported renal rBD1 and 88, 53, 53 and 50% identity with mouse, human, gorilla and rhesus monkey BD1 respectively. Furthermore, structural analysis of the cardiac rBD1 showed the classical six-cysteine conserved motif of the BD family with an alpha-helix and three beta-sheets. Additionally, rBD1 displayed a significantly greater number of amphoteric residues than any of the human analogs, indicating a strong pH functional dependence in the rat. We suggest that rBD1, which was initially believed to be a specific epithelium-derived peptide, may be also involved in local cardiac innate immune defense mechanisms. en_US
dc.language.iso en_US en_US
dc.relation.uri http://doi.org/10.3844/ajavsp.2008.1.6 en_US
dc.subject Innate immunity en_US
dc.subject Host defense peptides en_US
dc.subject Heart en_US
dc.subject Microdissection en_US
dc.title Rat cardiomyocytes express a classical epithelial beta-defensin en_US
dc.type Article (publisher version) en_US
dc.date.published 2008 en_US
dc.citation.doi 10.3844/ajavsp.2008.1.6 en_US
dc.citation.epage 6 en_US
dc.citation.issue 1 en_US
dc.citation.jtitle American Journal of Animal and Veterinary Sciences en_US
dc.citation.spage 1 en_US
dc.citation.volume 3 en_US
dc.contributor.authoreid alinde en_US
dc.contributor.authoreid blecha en_US
dc.contributor.authoreid tmelgare en_US

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