Continuous infusion of TNF alpha in adipose tissue does not induce the same metabolic effects as daily bolus injection in lactating dairy cows

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dc.contributor.author Martel, Cynthia Ann
dc.date.accessioned 2011-12-19T19:16:52Z
dc.date.available 2011-12-19T19:16:52Z
dc.date.issued 2011-12-19
dc.identifier.uri http://hdl.handle.net/2097/13261
dc.description.abstract Late-lactation Holstein cows (n=9/treatment) were used to evaluate effects of continuous adipose tissue TNFα administration on glucose and fatty acid (FA) metabolism. Cows were blocked by feed intake and milk yield and randomly assigned within block to control or TNFα treatments. Treatments (4 mL saline or 14 μg/kg TNFα in 4 mL saline) were infused continuously over 7 d via 2 osmotic pumps in the adipose layer in the tailhead region. Plasma, milk samples, milk yield, and dry matter intake (DMI) data were collected daily. On d 7, pumps were removed and liver and contralateral tailhead adipose biopsies were collected. Results were modeled with fixed effect of treatment and random effect of block; P values > 0.10 were considered non-significant. TNFα did not alter liver TNFα mRNA abundance, plasma TNFα, IL-4, IL-6, or interferon-γ concentrations, DMI, or rectal temperature. Milk fat and lactose concentrations decreased with TNFα (P < 0.05), but milk yield was unchanged and treatments did not alter the proportion of short vs. long-chain FA in milk on d 7. Treatments did not alter plasma NEFA concentration, liver triglyceride content, or adipose mRNA abundance for hormone-sensitive lipase or perilipin. Plasma glucose turnover rate, as measured by disappearance of U-13C-glucose bolus, was not altered by treatment, nor was liver mRNA abundance for phosphoenolpyruvate carboxykinase or pyruvate carboxylase. However, TNFα tended to decrease adipose TNFα mRNA abundance (P=0.09) and increase liver IL-10 mRNA abundance (P=0.05) compared to controls. Messenger RNA expression of IL-10 in adipose and IL-37 in liver tissue increased significantly in cows treated with TNFα (Figure 1; P = .02 adipose; P < 0.05 liver). This TNFα delivery protocol may have allowed for an adaptive anti-inflammatory response to suppress systemic inflammation, which may account for the lack of metabolic responses compared with previous responses to daily subcutaneous TNFα injections. en_US
dc.description.sponsorship This project was supported by National Research Initiative Competitive Grant no. 2009-35206-05271 from the USDA National Institute of Food and Agriculture. en_US
dc.language.iso en_US en_US
dc.publisher Kansas State University en
dc.subject Dairy en_US
dc.subject Gluconeogenesis en_US
dc.subject TNF-alpha en_US
dc.subject TRLP en_US
dc.title Continuous infusion of TNF alpha in adipose tissue does not induce the same metabolic effects as daily bolus injection in lactating dairy cows en_US
dc.type Thesis en_US
dc.description.degree Master of Science en_US
dc.description.level Masters en_US
dc.description.department Department of Human Nutrition en_US
dc.description.advisor Tonatiuh Melgarejo en_US
dc.subject.umi Agriculture, General (0473) en_US
dc.subject.umi Animal Sciences (0475) en_US
dc.date.published 2012 en_US
dc.date.graduationmonth December en_US


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