Conformational Changes and Catalytic Competency of Hydrolases Adsorbing on Fumed Silica Nanoparticles: II. Secondary Structure

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dc.contributor.author Cruz, Juan C.
dc.contributor.author Pfromm, Peter H.
dc.contributor.author Tomich, John M.
dc.contributor.author Rezac, Mary E.
dc.date.accessioned 2010-08-06T14:37:54Z
dc.date.available 2010-08-06T14:37:54Z
dc.date.issued 2010-08-06T14:37:54Z
dc.identifier.uri http://hdl.handle.net/2097/4486
dc.description.abstract Secondary conformational analysis via Circular Dichroism (CD) and Amide-I FTIR was applied to preparations of Candida antarctica Lipase B (CALB), subtilisin Carlsberg, and the Lipase from Thermomyces lanuginosus (TLL) on fumed silica to confirm that the “hardness” and packing density of the enzymes on the solid fumed silica nanoparticle surface can be used to rationalize the variable enzyme-dependent changes of catalytic competency with surface coverage. “Soft” enzymes should be immobilized at a surface coverage where enzyme-enzyme interactions predominate thereby preventing detrimental structural changes caused by enzyme-support interactions, while “hard” enzymes can be immobilized at low to intermediate surface coverage with good catalytic performance. Multi-layered coverage reduces the superficial average catalytic performance in all cases due to mass transfer limitations. en_US
dc.relation.uri http://www.sciencedirect.com en_US
dc.subject Conformational Stability en_US
dc.subject Adsorption en_US
dc.subject Fumed Silica en_US
dc.subject Protein-surface interactions en_US
dc.subject FTIR en_US
dc.subject CD en_US
dc.title Conformational Changes and Catalytic Competency of Hydrolases Adsorbing on Fumed Silica Nanoparticles: II. Secondary Structure en_US
dc.type Article (author version) en_US
dc.date.published 2010 en_US
dc.citation.doi doi:10.1016/j.colsurfb.2010.06.005 en_US
dc.citation.epage In press en_US
dc.citation.issue In press en_US
dc.citation.jtitle Colloids and Surfaces B: Biointerfaces en_US
dc.citation.spage In press en_US
dc.citation.volume In press en_US
dc.contributor.authoreid pfromm en_US
dc.contributor.authoreid rezac en_US
dc.contributor.authoreid jtomich en_US


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