Can the Gram-negative bacterium Escherichia coli colonize the gut of Lone Star Tick (Amblyomma americanum)?

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dc.contributor.author McKenzie, Taylor
dc.contributor.author Vanderree, Tessa
dc.contributor.author Maldonado-Ruiz, Paulina
dc.contributor.author Park, Yoonseong
dc.contributor.author Zurek, Ludek
dc.date.accessioned 2019-09-06T16:24:57Z
dc.date.available 2019-09-06T16:24:57Z
dc.date.issued 2018-12-14
dc.identifier.uri http://hdl.handle.net/2097/40185
dc.description.abstract Ticks are obligate blood feeding ectoparasites and vectors of several mammalian pathogens (Williams-Newkirk et al, 2014). In addition to pathogens they also carry a bacterial community with commensal and symbiotic relationships (Bonnet et al, 2017). Using a culture-dependent approach we previously reported a high prevalence of Gram-positive bacteria in the gut of field collected lone star ticks (Amblyomma americanum). These results suggested that epithelial immunity functions to control Gram-negative bacteria in A. americanum. In this study, we used a culturing and non-culturing approach to measure the outcome of E.coli (Gram-negative) when fed to female adult lone star ticks (n=16). Results showed a significant reduction of E.coli at Days 1, 3 and 7 post bacterial feeding. qPCR of 16S rDNA confirmed reduction of bacterial rDNA when compared to water fed ticks (n=16). Our results suggest that there is a midgut epithelial immune response in place, which mainly targets Gram-negative bacteria!
dc.language.iso en_US
dc.rights This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.rights.uri http://rightsstatements.org/vocab/InC/1.0/
dc.subject Fall 2018
dc.title Can the Gram-negative bacterium Escherichia coli colonize the gut of Lone Star Tick (Amblyomma americanum)?
dc.type Text
dc.date.published 2018
dc.citation.ctitle 3rd Entomology Undergraduate Research Poster Symposium. Manhattan, KS: Kansas State University, Department of Entomology.
dc.description.conference 3rd Entomology Undergraduate Research Poster Symposium. Manhattan, KS: Kansas State University, Department of Entomology.


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This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). Except where otherwise noted, the use of this item is bound by the following: This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

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