Effects of pH on Double Stranded RNA Stability in European Corn Borer Nucleases

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dc.contributor.author Biondi, Marie
dc.contributor.author Cooper, Anastasia
dc.contributor.author Song, Huifang
dc.contributor.author Yu, Zhitao
dc.contributor.author Zhu, Kun Yan
dc.date.accessioned 2019-08-23T19:36:21Z
dc.date.available 2019-08-23T19:36:21Z
dc.date.issued 2019
dc.identifier.uri http://hdl.handle.net/2097/40102
dc.description.abstract RNA interference (RNAI) is an immune response that can be exploited to make greener pesticides. It works by inciting suppression of a specific target gene using fed or injected dsRNA. Targeting a specific gene sequence also means RNAi can be used to target a specific organism. However, some insects, such as lepidopterans, have nucleases, called dsRNases, in their gut and hemolymph that sever dsRNA and lower RNAi efficiency (1). Ostrinia nubilalis, the European corn borer, (ECB), is a prime example of a lepidopteran pest which decimates corn supplies across the Midwest and does not respond to RNAi. Comparison of dsRNA stability in dsRNase genes in ECB and western corn rootworm (WCR), a coleopteran pest that has very high RNAi efficiency, indicates that dsRNA is rapidly degraded in ECB tissues, but not WCR tissues, despite similar expression of dsRNase genes in both species. These findings suggest that another variable, such as pH may be influencing dsRNA stability in insects (2).
dc.language.iso en_US
dc.rights This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.rights.uri http://rightsstatements.org/vocab/InC/1.0/
dc.subject 1st Plant Pathology REEU Poster Symposium, Summer 2019
dc.title Effects of pH on Double Stranded RNA Stability in European Corn Borer Nucleases
dc.type Text
dc.date.published 2019
dc.citation.ctitle 1st Plant Pathology REEU Poster Symposium, Summer 2019
dc.description.conference 1st Plant Pathology REEU Poster Symposium, Summer 2019


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This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). Except where otherwise noted, the use of this item is bound by the following: This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

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