Effects of EDTA feeding on survival and development of European corn borer larvae

K-REx Repository

Show simple item record

dc.contributor.author Knight, Mylah
dc.contributor.author Cooper, Anastasia
dc.contributor.author Silver, Kristopher
dc.date.accessioned 2018-09-26T22:24:59Z
dc.date.available 2018-09-26T22:24:59Z
dc.date.issued 2017-12-7
dc.identifier.uri http://hdl.handle.net/2097/39191
dc.description.abstract RNA interference (RNAi) is a process that uses double-stranded RNA (dsRNA) to target and suppress the expression of specific genes (Zhang et al., 2010). It is a powerful tool that has been used to understand the function of many genes and has great potential to control insect pests. However, differences in sensitivity to RNAi among insects have limited the use of RNAi (Kim et al., 2015). In some insects, such as European corn borer (ECB), RNAi efficiency is limited by degradation of dsRNA in insect gut. Strategies to overcome this limitation are necessary. Some studies have suggested that high temperatures or treatment with EDTA can inhibit nuclease activity (Garbutt et al., 2014), however the effects of treatment of insects with EDTA are unknown. The objective of this study was to determine if feeding different concentrations of EDTA to ECB larvae will have any effect on larval survival, weight, or development. Our results showed that neither 6 nor 10 mM EDTA had any significant effect on larval survival or development from 2nd instar to 3rd instar larvae. In contrast, average larval weight was suppressed by treatment with 10 mM EDTA. These data show that larvae can be safely treated with up to 6 mM EDTA without adverse effects on development or larval survival, suggesting that 6 mM EDTA can be used in future experiments to test the ability of EDTA to inhibit nuclease activity in ECB gut and possibly improve RNAi efficiency.
dc.language.iso en_US
dc.rights This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.rights.uri http://rightsstatements.org/vocab/InC/1.0/
dc.title Effects of EDTA feeding on survival and development of European corn borer larvae
dc.type Text
dc.date.published 2017
dc.citation.ctitle 2nd Entomology Undergraduate Research Poster Symposium. Manhattan, KS: Kansas State University, Department of Entomology.
dc.description.conference 2nd Entomology Undergraduate Research Poster Symposium. Manhattan, KS: Kansas State University, Department of Entomology.


Files in this item

This item appears in the following Collection(s)

Show simple item record

This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). Except where otherwise noted, the use of this item is bound by the following: This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

Search K-REx


Advanced Search

Browse

My Account

Statistics








Center for the

Advancement of Digital

Scholarship

118 Hale Library

Manhattan KS 66506


(785) 532-7444

cads@k-state.edu