GsMTx4 reduces the pressor response during dynamic hindlimb skeletal muscle stretch in decerebrate rats

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dc.contributor.author Sanderson, Bailey
dc.date.accessioned 2018-08-08T19:48:53Z
dc.date.available 2018-08-08T19:48:53Z
dc.date.issued 2018-08-01 en_US
dc.identifier.uri http://hdl.handle.net/2097/39112
dc.description.abstract Mechanical signals within contracting skeletal muscles contribute to the generation of the exercise pressor reflex; an important autonomic and cardiovascular control mechanism. In decerebrate rats, GsMTx4, a mechanically–activated channel inhibitor that is partially selective for piezo channels, was found recently to reduce the pressor response during static hindlimb muscle stretch; a maneuver used to investigate the mechanical component of the exercise pressor reflex (i.e., the mechanoreflex). However, the effect was found only during the very initial phase of the stretch when muscle length was changing which may have reflected the inhibition of rapidly-deactivating piezo 2 channels and the fact that different mechanically-activated channels with slower deactivation kinetics evoked the pressor response during the static phase of the maneuver. We tested the hypothesis that in decerebrate, unanesthetized rats, GsMTx4 would reduce the pressor response throughout the duration of a 30 second, 1 Hz dynamic hindlimb muscle stretch protocol. We found that the injection of 10 µg of GsMTx4 into the arterial supply of a hindlimb reduced the peak pressor response (control: 15±4, GsMTx4: 5±2 mmHg, p<0.05, n=8) and the pressor response at multiple time points throughout the duration of the stretch. GsMTx4, however, had no effect on the pressor response to the hindlimb arterial injection of lactic acid. Moreover, the injection of GsMTx4 into the jugular vein (a systemic control, n=5) or the injection of saline into the hindlimb arterial supply (a vehicle control, n=4) had no effect on the pressor response during dynamic stretch. We conclude that GsMTx4 reduced the pressor response throughout the duration of a 1 Hz dynamic stretch protocol which may have reflected the inhibition of piezo 2 channels throughout the dynamic stretch maneuver. en_US
dc.language.iso en_US en_US
dc.subject piezo channels en_US
dc.subject exercise pressor reflex en_US
dc.subject mechanoreflex en_US
dc.subject blood pressure en_US
dc.title GsMTx4 reduces the pressor response during dynamic hindlimb skeletal muscle stretch in decerebrate rats en_US
dc.type Thesis en_US
dc.description.degree Master of Science en_US
dc.description.level Masters en_US
dc.description.department Department of Kinesiology en_US
dc.description.advisor Steven W. Copp en_US
dc.date.published 2018 en_US
dc.date.graduationmonth August en_US


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