Prevalence of equine leptospiral shedding using urine polymerase chain reaction and serum microscopic agglutination testing

K-REx Repository

Show simple item record

dc.contributor.author Trimble, Amanda Carroll
dc.date.accessioned 2018-04-18T16:38:51Z
dc.date.available 2018-04-18T16:38:51Z
dc.date.issued 2018-05-01 en_US
dc.identifier.uri http://hdl.handle.net/2097/38800
dc.description.abstract Leptospirosis is a worldwide veterinary and public health concern, and emerging infectious disease of horses. The spirochete can be directly transmitted by contaminated urine, placental fluids, semen, infected tissues, reservoir hosts, or flood waters. Seroprevalence and infecting serovar vary with geography, yet diagnosis using the gold standard microscopic agglutination test (MAT) merely confirms a high exposure rate. Subclinical infection can complicate diagnosis. The aims of this study were to use semi-nested PCR on urine from apparently healthy horses to determine period prevalence of leptospiral shedding and to correlate these findings with MAT results to establish associations with client based survey data regarding horse management and environment. Serum and free-catch urine were collected from 204 healthy horses between May 2016- December 2017. Serum was used to determine GGT, creatinine concentrations, and six serovar MAT (Canicola, Hardjo, Icterhemorrhaegiae, Pomona, Grippotyphosa, Bratislava). Urine samples were submitted for PCR testing of leptospiral 23S rRNA. Client consent and survey data were collected for all subjects. Potential risk factors included drinking water source, exposure to livestock and dogs, geographical location, season, and precipitation. Two horses were positive on urine PCR for leptospirosis (shedding prevalence 1%), yet only one had a high reciprocal MAT titer of ≥800. Both horses were negative on urine PCR one month later without treatment. Approximately 77% of horses (157/204) were seroreactive (MAT reciprocal titer ≥100) for at least one serogroup, and Bratislava was detected more frequently than others (47.5%; (97/204)). Apparently healthy horses infrequently shed Leptospira spp. in urine, yet seroreactivity in clinically normal horses is high (77%), confirming high exposure rates to Leptospira spp. in the Central Midwest. Further studies should target serovar specific PCR tests and incorporate PCR testing in horses clinically affected with leptospirosis. en_US
dc.description.sponsorship Department of Clinical Sciences (KSU)​; Dr. John G. and Mildred L. Gish Research Fund for Large Animal Research ; Zoetis en_US
dc.language.iso en_US en_US
dc.subject Equine en_US
dc.subject Leptospirosis en_US
dc.title Prevalence of equine leptospiral shedding using urine polymerase chain reaction and serum microscopic agglutination testing en_US
dc.title.alternative Prevalence of equine leptospiral shedding using urine polymerase chain reaction (PCR) and serum microagglutination testing (MAT) en_US
dc.type Thesis en_US
dc.description.degree Master of Science in Biomedical Sciences en_US
dc.description.level Masters en_US
dc.description.department Department of Clinical Sciences en_US
dc.description.advisor Elizabeth Davis en_US
dc.date.published 2018 en_US
dc.date.graduationmonth May en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search K-REx


Advanced Search

Browse

My Account

Statistics








Center for the

Advancement of Digital

Scholarship

cads@k-state.edu