Integrated bioprocess to boost cellulosic bioethanol titers and yields

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dc.contributor.author Xu, Youjie
dc.date.accessioned 2018-04-09T21:25:14Z
dc.date.available 2018-04-09T21:25:14Z
dc.date.issued 2018-05-01 en_US
dc.identifier.uri http://hdl.handle.net/2097/38754
dc.description.abstract Among potential alternative liquid fuels, bioethanol is the widest utilized transportation fuels and mainly made from grains. Cellulosic biofuels provide environmental benefits not available from grain or sugar-based biofuels and are considered as a solid foundation to meet transportation fuels needs in a low-carbon economy, albeit with electrified vehicles and other technical advances. The objective of this research was to develop and optimize various bioprocessing units to boost cellulosic bioethanol titers and yields in order to accelerate the commercialization of cellulosic bioethanol production. The results showed high-solids biomass bioconversion (12%, w/v) was inefficient in the laboratory rotary shaker. However, a horizontal reactor with good mixing was effective for high solids loading (20%, w/v), yielding 75 g/L of glucose. To achieve the minimal economical ethanol distillation requirement of 40 g/L, integrated bioprocesses were conducted to boost ethanol titers and yields through co-fermentation of starchy grain and cellulosic biomass. The maximum ethanol concentration (68.7 g/L) was achieved at the corn flour and hydrothermal-treated corn stover ratio of 12:12 using raw starch granular enzyme with the ethanol yield of 86.0%. Co-fermentation of starchy substrate with hydrolysate liquor from saccharified biomass was able to significantly enhance ethanol concentration and reduce energy cost for distillation without sacrificing ethanol yields. These results indicated integration of first and second generation ethanol production could significantly accelerate the commercialization of cellulosic biofuel production. Novel technology, modified simultaneous saccharification and fermentation, was firstly established to enhance ethanol titers and yields, which achieved high ethanol titers of 72.3 g/L at high biomass loadings of 30% (w/v) with 70.0% ethanol yield. en_US
dc.language.iso en_US en_US
dc.publisher Kansas State University en
dc.subject Biomass en_US
dc.subject Biofuels en_US
dc.subject Pretreatment en_US
dc.subject Fermentation en_US
dc.subject Ethanol en_US
dc.title Integrated bioprocess to boost cellulosic bioethanol titers and yields en_US
dc.type Dissertation en_US
dc.description.degree Doctor of Philosophy en_US
dc.description.level Doctoral en_US
dc.description.department Department of Biological & Agricultural Engineering en_US
dc.description.advisor Donghai Wang en_US
dc.date.published 2018 en_US
dc.date.graduationmonth May en_US


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