Detection of extremely low concentration waterborne pathogen using a multiplexing self-referencing SERS microfluidic biosensor

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dc.contributor.author Wang, C.
dc.contributor.author Madiyar, F.
dc.contributor.author Yu, C. X.
dc.contributor.author Li, Jun
dc.date.accessioned 2017-11-30T21:44:08Z
dc.date.available 2017-11-30T21:44:08Z
dc.date.issued 2017-02-14
dc.identifier.uri http://hdl.handle.net/2097/38355
dc.description Citation: Wang, C., Madiyar, F., Yu, C. X., & Li, J. (2017). Detection of extremely low concentration waterborne pathogen using a multiplexing self-referencing SERS microfluidic biosensor. Journal of Biological Engineering, 11, 11. doi:10.1186/s13036-017-0051-x
dc.description.abstract Background: It is challenging to achieve ultrasensitive and selective detection of waterborne pathogens at extremely low levels (i.e., single cell/mL) using conventional methods. Even with molecular methods such as ELISA or PCR, multi-enrichment steps are needed which are labor and cost intensive. In this study, we incorporated nano-dielectrophoretic microfluidic device with Surface enhanced Raman scattering (SERS) technique to build a novel portable biosensor for easy detection and characterization of Escherichia coli O157:H7 at high sensitivity level (single cell/mL). Results: A multiplexing dual recognition SERS scheme was developed to achieve one-step target detection without the need to separate target-bound probes from unbound ones. With three different SERS-tagged molecular probes targeting different epitopes of the same pathogen being deployed simultaneously, detection of pathogen targets was achieved at single cell level with sub-species specificity that has not been reported before in single-step pathogen detection. Conclusion: The self-referencing protocol implements with a Nano-dielectrophoretic microfluidic device potentially can become an easy-to-use, field-deployable spectroscopic sensor for onsite detection of pathogenic microorganisms.
dc.relation.uri https://doi.org/10.1186/s13036-017-0051-x
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Enhanced Raman-Spectroscopy
dc.subject Label-Free Sers
dc.subject Bacteria
dc.subject Dielectrophoresis
dc.subject Microorganisms
dc.subject Identification
dc.title Detection of extremely low concentration waterborne pathogen using a multiplexing self-referencing SERS microfluidic biosensor
dc.type Article
dc.date.published 2017
dc.citation.doi 10.1186/s13036-017-0051-x
dc.citation.issn 1754-1611
dc.citation.jtitle Journal of Biological Engineering
dc.citation.spage 11
dc.citation.volume 11
dc.citation Wang, C., Madiyar, F., Yu, C. X., & Li, J. (2017). Detection of extremely low concentration waterborne pathogen using a multiplexing self-referencing SERS microfluidic biosensor. Journal of Biological Engineering, 11, 11. doi:10.1186/s13036-017-0051-x
dc.contributor.authoreid junli
dc.contributor.kstate Li, Jun


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