Archaea and Bacteria Acclimate to High Total Ammonia in a Methanogenic Reactor Treating Swine Waste

K-REx Repository

Show simple item record

dc.contributor.author Esquivel-Elizondo, S.
dc.contributor.author Parameswaran, P.
dc.contributor.author Delgado, A. G.
dc.contributor.author Maldonado, J.
dc.contributor.author Rittmann, B. E.
dc.contributor.author Krajmalnik-Brown, R.
dc.date.accessioned 2017-11-30T21:43:28Z
dc.date.available 2017-11-30T21:43:28Z
dc.date.issued 2016-09-20
dc.identifier.uri http://hdl.handle.net/2097/38346
dc.description Citation: Esquivel-Elizondo, S., Parameswaran, P., Delgado, A. G., Maldonado, J., Rittmann, B. E., & Krajmalnik-Brown, R. (2016). Archaea and Bacteria Acclimate to High Total Ammonia in a Methanogenic Reactor Treating Swine Waste. Archaea-an International Microbiological Journal, 10. doi:10.1155/2016/4089684
dc.description.abstract Inhibition by ammonium at concentrations above 1000mgN/L is known to harm the methanogenesis phase of anaerobic digestion. We anaerobically digested swine waste and achieved steady state COD-removal efficiency of around 52% with no fatty-acid or H-2 accumulation. As the anaerobic microbial community adapted to the gradual increase of total ammonia-N (NH3 -N) from 890 +/- 295 to 2040 +/- 30 mg/L, the Bacterial and Archaeal communities became less diverse. Phylotypes most closely related to hydrogenotrophic Methanoculleus (36.4%) and Methanobrevibacter (11.6%), along with acetoclastic Methanosaeta (29.3%), became the most abundant Archaeal sequences during acclimation. This was accompanied by a sharp increase in the relative abundances of phylotypes most closely related to acetogens and fatty-acid producers (Clostridium, Coprococcus, and Sphaerochaeta) and syntrophic fatty-acid Bacteria (Syntrophomonas, Clostridium, Clostridiaceae species, and Cloacamonaceae species) that have metabolic capabilities for butyrate and propionate fermentation, as well as for reverse acetogenesis. Our results provide evidence countering a prevailing theory that acetoclastic methanogens are selectively inhibited when the total ammonia-N concentration is greater than similar to 1000 mgN/L. Instead, acetoclastic and hydrogenotrophic methanogens coexisted in the presence of total ammonia-N of similar to 2000 mgN/L by establishing syntrophic relationships with fatty-acid fermenters, as well as homoacetogens able to carry out forward and reverse acetogenesis.
dc.relation.uri https://doi.org/10.1155/2016/4089684
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Complete Genome Sequence
dc.subject 16S Ribosomal-Rna
dc.subject Microbial Communities
dc.subject Acetate Oxidation
dc.subject Propionate Oxidation
dc.subject Oxidizing Bacterium
dc.title Archaea and Bacteria Acclimate to High Total Ammonia in a Methanogenic Reactor Treating Swine Waste
dc.type Text
dc.date.published 2016
dc.citation.doi 10.1155/2016/4089684
dc.citation.issn 1472-3646
dc.citation.jtitle Archaea-an International Microbiological Journal
dc.citation.spage 10
dc.contributor.authoreid prathapp
dc.description.version Article: Version of Record
dc.contributor.kstate Parameswaran, Prathap


Files in this item

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International (CC BY 4.0) Except where otherwise noted, the use of this item is bound by the following: Attribution 4.0 International (CC BY 4.0)

Search K-REx


Advanced Search

Browse

My Account

Statistics








Center for the

Advancement of Digital

Scholarship

cads@k-state.edu