Genomic signatures of Mannheimia haemolytica that associate with the lungs of cattle with respiratory disease, an integrative conjugative element, and antibiotic resistance genes

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dc.contributor.author Clawson, M. L.
dc.contributor.author Murray, R. W.
dc.contributor.author Sweeney, M. T.
dc.contributor.author Apley, Michael D.
dc.contributor.author DeDonder, Keith D.
dc.contributor.author Capik, Sarah F.
dc.contributor.author Larson, Robert L.
dc.contributor.author Lubbers, Brian V.
dc.contributor.author White, Bradley J.
dc.contributor.author Kalbfleisch, T. S.
dc.contributor.author Schuller, G.
dc.contributor.author Dickey, A. M.
dc.contributor.author Harhay, G. P.
dc.contributor.author Heaton, M. P.
dc.contributor.author Chitko-McKown, C. G.
dc.contributor.author Brichta-Harhay, D. M.
dc.contributor.author Bono, J. L.
dc.contributor.author Smith, T. P. L.
dc.date.accessioned 2017-11-30T21:43:15Z
dc.date.available 2017-11-30T21:43:15Z
dc.identifier.uri http://hdl.handle.net/2097/38344
dc.description Citation: Clawson, M. L., Murray, R. W., Sweeney, M. T., Apley, M. D., DeDonder, K. D., Capik, S. F., . . . Smith, T. P. L. (2016). Genomic signatures of Mannheimia haemolytica that associate with the lungs of cattle with respiratory disease, an integrative conjugative element, and antibiotic resistance genes. Bmc Genomics, 17, 14. doi:10.1186/s12864-016-3316-8
dc.description.abstract Background: Mannheimia haemolytica typically resides in cattle as a commensal member of the upper respiratory tract microbiome. However, some strains can invade their lungs and cause respiratory disease and death, including those with multi-drug resistance. A nucleotide polymorphism typing system was developed for M. haemolytica from the genome sequences of 1133 North American isolates, and used to identify genetic differences between isolates from the lungs and upper respiratory tract of cattle with and without clinical signs of respiratory disease. Results: A total of 26,081 nucleotide polymorphisms were characterized after quality control filtering of 48,403 putative polymorphisms. Phylogenetic analyses of nucleotide polymorphism genotypes split M. haemolytica into two major genotypes (1 and 2) that each were further divided into multiple subtypes. Multiple polymorphisms were identified with alleles that tagged genotypes 1 or 2, and their respective subtypes. Only genotype 2 M. haemolytica associated with the lungs of diseased cattle and the sequence of a particular integrative and conjugative element (ICE). Additionally, isolates belonging to one subtype of genotype 2 (2b), had the majority of antibiotic resistance genes detected in this study, which were assorted into seven combinations that ranged from 1 to 12 resistance genes. Conclusions: Typing of diverse M. haemolytica by nucleotide polymorphism genotypes successfully identified associations with diseased cattle lungs, ICE sequence, and antibiotic resistance genes. Management of cattle by their carriage of M. haemolytica could be an effective intervention strategy to reduce the prevalence of respiratory disease and supplemental needs for antibiotic treatments in North American herds.
dc.relation.uri https://doi.org/10.1186/s12864-016-3316-8
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Mannheimia
dc.subject Haemolytica
dc.subject Genomics
dc.subject Polymorphisms
dc.subject Genotypes
dc.subject Subtypes
dc.title Genomic signatures of Mannheimia haemolytica that associate with the lungs of cattle with respiratory disease, an integrative conjugative element, and antibiotic resistance genes
dc.type Article
dc.date.published 2016
dc.citation.doi 10.1186/s12864-016-3316-8
dc.citation.issn 1471-2164
dc.citation.jtitle Bmc Genomics
dc.citation.spage 14
dc.citation.volume 17
dc.contributor.authoreid mapley
dc.contributor.authoreid whiteb
dc.contributor.authoreid rlarson
dc.contributor.authoreid blubbers
dc.contributor.kstate Apley, Michael D.
dc.contributor.kstate White, Bradley J.
dc.contributor.kstate Larson, Robert L.
dc.contributor.kstate Lubbers, Brian V.


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Attribution 4.0 International (CC BY 4.0) Except where otherwise noted, the use of this item is bound by the following: Attribution 4.0 International (CC BY 4.0)

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