Divergence of the diapause transcriptome in apple maggot flies: winter regulation and post-winter transcriptional repression

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dc.contributor.author Meyers, P. J.
dc.contributor.author Powell, T. H. Q.
dc.contributor.author Walden, K. K. O.
dc.contributor.author Schieferecke, Adam J.
dc.contributor.author Feder, J. L.
dc.contributor.author Hahn, D. A.
dc.contributor.author Robertson, H. M.
dc.contributor.author Berlocher, S. H.
dc.contributor.author Ragland, Gregory J.
dc.date.accessioned 2017-11-30T21:42:36Z
dc.date.available 2017-11-30T21:42:36Z
dc.identifier.uri http://hdl.handle.net/2097/38336
dc.description Citation: Meyers, P. J., Powell, T. H. Q., Walden, K. K. O., Schieferecke, A. J., Feder, J. L., Hahn, D. A., . . . Ragland, G. J. (2016). Divergence of the diapause transcriptome in apple maggot flies: winter regulation and post-winter transcriptional repression. Journal of Experimental Biology, 219(17), 2613-2622. doi:10.1242/jeb.140566
dc.description.abstract The duration of dormancy regulates seasonal timing in many organisms and may be modulated by day length and temperature. Though photoperiodic modulation has been well studied, temperature modulation of dormancy has received less attention. Here, we leverage genetic variation in diapause in the apple maggot fly, Rhagoletis pomonella, to test whether gene expression during winter or following spring warming regulates diapause duration. We used RNAseq to compare transcript abundance during and after simulated winter between an apple-infesting population and a hawthorn-infesting population where the apple population ends pupal diapause earlier than the hawthorn-infesting population. Marked differences in transcription between the two populations during winter suggests that the 'early' apple population is developmentally advanced compared with the 'late' hawthorn population prior to spring warming, with transcripts participating in growth and developmental processes relatively up-regulated in apple pupae during the winter cold period. Thus, regulatory differences during winter ultimately drive phenological differences that manifest themselves in the following summer. Expression and polymorphism analysis identify candidate genes in the Wnt and insulin signaling pathways that contribute to population differences in seasonality. Both populations remained in diapause and displayed a pattern of up-and then down-regulation (or vice versa) of growth-related transcripts following warming, consistent with transcriptional repression. The ability to repress growth stimulated by permissive temperatures is likely critical to avoid mismatched phenology and excessive metabolic demand. Compared with diapause studies in other insects, our results suggest some overlap in candidate genes/pathways, though the timing and direction of changes in transcription are likely species specific.
dc.relation.uri https://doi.org/10.1242/jeb.140566
dc.rights This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.rights.uri http://jeb.biologists.org/content/rights-permissions
dc.subject Diapause
dc.subject Phenology
dc.subject Overwintering
dc.subject Gene Expression
dc.subject Rhagoletis
dc.subject Pitcher-Plant Mosquito
dc.title Divergence of the diapause transcriptome in apple maggot flies: winter regulation and post-winter transcriptional repression
dc.type Article
dc.date.published 2016
dc.citation.doi 10.1242/jeb.140566
dc.citation.epage 2622
dc.citation.issn 0022-0949
dc.citation.issue 17
dc.citation.jtitle Journal of Experimental Biology
dc.citation.spage 2613
dc.citation.volume 219
dc.description.embargo 2017-12
dc.contributor.authoreid gragland
dc.contributor.kstate Ragland, Gregory J.

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