Molecular Landscape of the Ribosome Pre-initiation Complex during mRNA Scanning: Structural Role for eIF3c and Its Control by eIF5

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dc.contributor.author Obayashi, E.
dc.contributor.author Luna, R. E.
dc.contributor.author Nagata, T.
dc.contributor.author Martin-Marcos, P.
dc.contributor.author Hiraishi, H.
dc.contributor.author Singh, Chingakham Ranjit
dc.contributor.author Erzberger, J. P.
dc.contributor.author Zhang, F.
dc.contributor.author Arthanari, H.
dc.contributor.author Morris, J.
dc.contributor.author Pellarin, R.
dc.contributor.author Moore, C.
dc.contributor.author Harmon, I.
dc.contributor.author Papadopoulos, E.
dc.contributor.author Yoshida, H.
dc.contributor.author Nasr, M. L.
dc.contributor.author Unzai, S.
dc.contributor.author Thompson, B.
dc.contributor.author Aube, E.
dc.contributor.author Hustak, S.
dc.contributor.author Stengel, F.
dc.contributor.author Dagraca, E.
dc.contributor.author Ananbandam, A.
dc.contributor.author Gao, P.
dc.contributor.author Urano, T.
dc.contributor.author Hinnebusch, A. G.
dc.contributor.author Wagner, G.
dc.contributor.author Asano, Katsura
dc.date.accessioned 2017-11-30T21:40:25Z
dc.date.available 2017-11-30T21:40:25Z
dc.identifier.uri http://hdl.handle.net/2097/38323
dc.description Citation: Obayashi, E., Luna, R. E., Nagata, T., Martin-Marcos, P., Hiraishi, H., Singh, C. R., . . . Asano, K. (2017). Molecular Landscape of the Ribosome Pre-initiation Complex during mRNA Scanning: Structural Role for eIF3c and Its Control by eIF5. Cell Reports, 18(11), 2651-2663. doi:10.1016/j.celrep.2017.02.052
dc.description.abstract During eukaryotic translation initiation, eIF3 binds the solvent-accessible side of the 40S ribosome and recruits the gate-keeper protein eIF1 and eIF5 to the decoding center. This is largely mediated by the N-terminal domain (NTD) of eIF3c, which can be divided into three parts: 3c0, 3c1, and 3c2. The N-terminal part, 3c0, binds eIF5 strongly but only weakly to the ribosome-binding surface of eIF1, whereas 3c1 and 3c2 form a stoichiometric complex with eIF1. 3c1 contacts eIF1 through Arg-53 and Leu-96, while 3c2 faces 40S protein uS15/S13, to anchor eIF1 to the scanning pre-initiation complex (PIC). We propose that the 3c0:eIF1 interaction diminishes eIF1 binding to the 40S, whereas 3c0:eIF5 interaction stabilizes the scanning PIC by precluding this inhibitory interaction. Upon start codon recognition, interactions involving eIF5, and ultimately 3c0:eIF1 association, facilitate eIF1 release. Our results reveal intricate molecular interactions within the PIC, programmed for rapid scanning-arrest at the start codon.
dc.relation.uri https://doi.org/10.1016/j.celrep.2017.02.052
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Eukaryotic Translation Initiation
dc.subject Start-Codon Recognition
dc.subject Selection
dc.subject In-Vivo
dc.subject C-Terminal Domain
dc.subject Preinitiation Complex
dc.title Molecular Landscape of the Ribosome Pre-initiation Complex during mRNA Scanning: Structural Role for eIF3c and Its Control by eIF5
dc.type Article
dc.date.published 2017
dc.citation.doi 10.1016/j.celrep.2017.02.052
dc.citation.epage 2663
dc.citation.issn 2211-1247
dc.citation.issue 11
dc.citation.jtitle Cell Reports
dc.citation.spage 2651
dc.citation.volume 18
dc.contributor.authoreid csingh
dc.contributor.authoreid kasano
dc.contributor.kstate Singh, Chingakham Ranjit
dc.contributor.kstate Asano, Katsura


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