Experimental and modeling study of a membrane filtration process using ceramic membranes to increase retroviral pseudotype vector titer

K-REx Repository

Show simple item record

dc.contributor.author Nehring, Dirk
dc.contributor.author Gonzalez, Roberto
dc.contributor.author Czermak, Peter
dc.contributor.author Pörtner, Ralf
dc.date.accessioned 2010-04-12T18:30:04Z
dc.date.available 2010-04-12T18:30:04Z
dc.date.issued 2004-07-01
dc.identifier.uri http://hdl.handle.net/2097/3574
dc.description.abstract The ability of commercial ceramic asymmetric ultrafiltration membranes with a cut-off of 10 and 20kDa to purify retroviral pseudotype vectors derived from the murine leukemia virus carrying the HIV 1 envelop protein MLV(HIV-1) was studied. To optimize the filtration process a mathematical model of batch wise vector purification was set up. 745 to 1794ml batches of supernatant containing a maximum of 3.2 x 10[superscript]5 colony forming units per ml (cfu/ml) was produced in a 200 ml fixed bed reactor. By cross flow filtration the vector concentration was increased 10-fold with an average recovery of 84.5 ± 4.5 % of the initial infective capacity. Furthermore membrane layer formation and temperature dependent decay of transduction competent vector particles (decay) was included in the mathematical model. A maximal end point titer of 4.1 x 10[superscript]6 cfu/ml was predicted by the model and confirmed reasonably well by experimental data. Transmembrane flow of batch filtration was predicted by solving a set of related differential equations. Our modeling allows scale-up of the process and prediction of process performance including specific issues such as vector degradation. en_US
dc.relation.uri http://doi.org/10.1016/j.memsci.2004.02.027 en_US
dc.rights This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.rights.uri https://rightsstatements.org/page/InC/1.0/
dc.subject Retrovirus en_US
dc.subject Gene therapy en_US
dc.subject Purification en_US
dc.subject Membrane filtration en_US
dc.subject Layer formation en_US
dc.subject Mathematical model en_US
dc.subject Ceramic membrane en_US
dc.title Experimental and modeling study of a membrane filtration process using ceramic membranes to increase retroviral pseudotype vector titer en_US
dc.type Text en_US
dc.date.published 2004 en_US
dc.citation.doi 10.1016/j.memsci.2004.02.027 en_US
dc.citation.epage 38 en_US
dc.citation.issue 1-2 en_US
dc.citation.jtitle Journal of Membrane Science en_US
dc.citation.spage 25 en_US
dc.citation.volume 237 en_US
dc.contributor.authoreid pczermak en_US
dc.description.version Article (author version)


Files in this item

This item appears in the following Collection(s)

Show simple item record

This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). Except where otherwise noted, the use of this item is bound by the following: This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

Search K-REx


Advanced Search

Browse

My Account

Statistics








Center for the

Advancement of Digital

Scholarship

cads@k-state.edu