Evaluation of individual and combined antimicrobial spray treatments on chilled beef subprimal cuts to reduce Shiga toxin-producing Escherichia coli populations

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dc.contributor.author Acuff, Jennifer Claire
dc.date.accessioned 2017-04-21T20:49:33Z
dc.date.available 2017-04-21T20:49:33Z
dc.date.issued 2017-05-01 en_US
dc.identifier.uri http://hdl.handle.net/2097/35504
dc.description.abstract Due to the potential of Shiga toxin-producing Escherichia coli (STEC) contamination, beef processors use various antimicrobial interventions throughout the slaughter and fabrication processes to reduce risks of contaminating the food supply. Certain antimicrobials are approved and marketed for spraying onto chilled subprimal cuts; however, administering these treatments through commercial-scale equipment against foodborne pathogens is not fully validated. This study evaluated the efficacy of three common antimicrobial sprays, individually (Study 1) and combined (Study 2), against a rifampicin-resistant STEC cocktail (O26, O45, O103, O111, O121, O145, and O157:H7) using a commercial style subprimal spray cabinet. For Study 1, beef subprimals (n=16) were mist-inoculated with the cocktail (ca. 5 log CFU/cm²), followed by spray-treatment with individual antimicrobials [200 ppm peracetic acid (PAA), 2% Centron™ (sulfuric acid, sodium sulfate anhydrous and water mixture; CEN), 4.5% lactic acid (LA), or water (W)]. Study 1 was designed as randomized generalized block. After each treatment phase, STEC population reductions were quantified. As individual antimicrobial treatments, LA and PAA provided greater (P ≤ 0.05) STEC reductions (0.5 and 0.6 ± 0.08 log CFU/cm², respectively) compared to water (0.2 ± 0.08 log CFU/cm²), but the CEN reduction (0.4 ± 0.10 log CFU/cm²) was statistically similar to W. To test the efficacy of combined treatments on subprimal cuts in Study 2, a split-plot design was used using three replications. The inoculated subprimals (n=4) were first treated with PAA, LA, CEN, or W; vacuum packaged; and stored for 72 hours at 4°C. Each subprimal was then divided (n=16) and treated with each of the four antimicrobials as a second treatment. Cumulative reductions from the two treatments and storage ranged from 0.5 to 1.5 log CFU/cm² (± 0.3 log CFU/cm²); the greatest reduction was observed when subprimals were treated with LA followed by vacuum packaged storage and another LA application. Nevertheless, there was no statistical significance among treatments for a particular combination of treatments in Study 2. These studies indicate that the individual antimicrobial treatments evaluated are marginally effective for reducing STEC populations on chilled beef subprimal cuts during fabrication. Although there does not seem to be a specific combination of treatment that is more effective than another, the overall bacterial reduction may be improved by combining treatments when the beef is stored under vacuum packaged conditions and retreated upon bag opening, as typical of mechanical tenderization operations. en_US
dc.description.sponsorship United States Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative Shiga Toxin-Producing Escherichia coli Coordinated Agricultural Project Grant en_US
dc.publisher Kansas State University en
dc.subject Antimicrobials en_US
dc.subject Beef subprimal en_US
dc.subject Lactic acid en_US
dc.subject Peracetic acid en_US
dc.subject Interventions en_US
dc.title Evaluation of individual and combined antimicrobial spray treatments on chilled beef subprimal cuts to reduce Shiga toxin-producing Escherichia coli populations en_US
dc.type Thesis en_US
dc.description.degree Master of Science en_US
dc.description.level Masters en_US
dc.description.department Food Science Institute en_US
dc.description.advisor Randall K. Phebus en_US
dc.date.published 2017 en_US
dc.date.graduationmonth May en_US

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