Elimination of Porcine Epidemic Diarrhea Virus in an Animal Feed Manufacturing Facility

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dc.contributor.author Huss, Anne R.
dc.contributor.author Schumacher, Loni L.
dc.contributor.author Cochrane, Roger A.
dc.contributor.author Poulsen, Elizabeth
dc.contributor.author Bai, Jianfa
dc.contributor.author Woodworth, Jason C.
dc.contributor.author Dritz, Steve S.
dc.contributor.author Stark, Charles R.
dc.contributor.author Jones, Cassandra K.
dc.date.accessioned 2017-04-07T16:53:53Z
dc.date.available 2017-04-07T16:53:53Z
dc.date.issued 2017-01-18
dc.identifier.uri http://hdl.handle.net/2097/35302
dc.description Citation: Huss AR, Schumacher LL, Cochrane RA, Poulsen E, Bai J, Woodworth JC, et al. (2017) Elimination of Porcine Epidemic Diarrhea Virus in an Animal Feed Manufacturing Facility. PLoS ONE 12(1): e0169612. doi:10.1371/journal.pone.0169612
dc.description.abstract Porcine Epidemic Diarrhea Virus (PEDV) was the first virus of wide scale concern to be linked to possible transmission by livestock feed or ingredients. Measures to exclude pathogens, prevent cross-contamination, and actively reduce the pathogenic load of feed and ingredients are being developed. However, research thus far has focused on the role of chemicals or thermal treatment to reduce the RNA in the actual feedstuffs, and has not addressed potential residual contamination within the manufacturing facility that may lead to continuous contamination of finished feeds. The purpose of this experiment was to evaluate the use of a standardized protocol to sanitize an animal feed manufacturing facility contaminated with PEDV. Environmental swabs were collected throughout the facility during the manufacturing of a swine diet inoculated with PEDV. To monitor facility contamination of the virus, swabs were collected at: 1) baseline prior to inoculation, 2) after production of the inoculated feed, 3) after application of a quaternary ammonium-glutaraldehyde blend cleaner, 4) after application of a sodium hypochlorite sanitizing solution, and 5) after facility heat-up to 60°C for 48 hours. Decontamination step, surface, type, zone and their interactions were all found to impact the quantity of detectable PEDV RNA (P < 0.05). As expected, all samples collected from equipment surfaces contained PEDV RNA after production of the contaminated feed. Additionally, the majority of samples collected from non-direct feed contact surfaces were also positive for PEDV RNA after the production of the contaminated feed, emphasizing the potential role dust plays in cross-contamination of pathogen throughout a manufacturing facility. Application of the cleaner, sanitizer, and heat were effective at reducing PEDV genomic material (P < 0.05), but did not completely eliminate it.
dc.relation.uri http://dx.doi.org/10.1371/journal.pone.0169612
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Decontamination
dc.subject Equipment
dc.subject Rubber
dc.subject Hypochlorites
dc.subject Swine
dc.subject Diet
dc.title Elimination of Porcine Epidemic Diarrhea Virus in an Animal Feed Manufacturing Facility
dc.type Article
dc.date.published 2017
dc.citation.doi 10.1371/journal.pone.0169612
dc.citation.issn 1932-6203
dc.citation.issue 1
dc.citation.jtitle PLoS ONE
dc.citation.volume 12
dc.contributor.authoreid jbai
dc.contributor.authoreid jwoodworth
dc.contributor.authoreid dritz
dc.contributor.authoreid crstark
dc.contributor.authoreid jonesc
dc.contributor.kstate Huss, Anne R.
dc.contributor.kstate Schumacher, Loni L.
dc.contributor.kstate Cochrane, Roger A.
dc.contributor.kstate Poulsen, Elizabeth
dc.contributor.kstate Bai, Jianfa
dc.contributor.kstate Woodworth, Jason C.
dc.contributor.kstate Dritz, Steve S.
dc.contributor.kstate Stark, Charles R.
dc.contributor.kstate Jones, Cassandra K.


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