Evaluation of Several Genes as Targets for RNAi in German Cockroach

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dc.contributor.author Wilkins, Rachel
dc.contributor.author Zhu, Kun Yan
dc.contributor.author Silver, Kristopher
dc.date.accessioned 2017-02-20T19:31:24Z
dc.date.available 2017-02-20T19:31:24Z
dc.identifier.uri http://hdl.handle.net/2097/35253
dc.description Citation: Wilkins, R. (2017). Evaluation of Several Genes as Targets for RNAi in German Cockroach . 1st Annual Undergraduate Research Experience in Entomology Symposium, November 16, 2016. Manhattam, KS.
dc.description.abstract RNA interference (RNAi) was first discovered in nematodes when exogenous double-stranded RNA (dsRNA) complementary to a specific gene suppressed expression of that gene [1]. Subsequently, much effort has been devoted to developing RNAi as a highly specific tool for therapeutic interventions and control of insect pests [2]. However, there are still many challenges associated with using RNAi to control insects, including efficient delivery and selection of appropriate targets. In this study, we evaluated three genes as potential targets for causing mortality via RNAi in German cockroach, Blatella germanica. German cockroaches are ubiquitous structural pests that can serve as reservoirs for species of pathogenic bacteria, viruses, or fungi in humans [3,4] and is an excellent organism for exploring insect control using RNAi. Injection of dsRNA complementary to either tubulin (Tub, a cytoskeletal structural protein), VATPase subunit 1 (Vha, an integral membrane protein), or Snf7 (an ESCRT III protein) caused decreased survival with Snf7 causing the greatest and fastest mortality (LT50 = 8.2 days). Our results demonstrate that when suppressed with RNAi, these genes could be effective targets for cockroach control. Furthermore, knowing these genes can be effectively used for RNAi, we can now attempt to understand why methods other than injection for RNAi delivery are less efficient in an effort to improve the utility of RNAi in insect control.
dc.language.iso en_US
dc.rights This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
dc.rights.uri http://rightsstatements.org/vocab/InC/1.0/
dc.title Evaluation of Several Genes as Targets for RNAi in German Cockroach
dc.type Text
dc.date.published 2017
dc.citation.ctitle 1st Annual Undergraduate Research Experience in Entomology Symposium, November 16, 2016. Manhattam, KS.
dc.description.conference 1st Annual Undergraduate Research Experience in Entomology Symposium, November 16, 2016. Manhattam, KS.
dc.contributor.authoreid rvw
dc.contributor.kstate Wilkins, Rachel
dc.contributor.kstate Zhu, Kun Yan
dc.contributor.kstate Silver, Kristopher


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This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). Except where otherwise noted, the use of this item is bound by the following: This Item is protected by copyright and/or related rights. You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).

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