A novel role for poly(C) binding proteins in programmed ribosomal frameshifting

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dc.contributor.author Napthine, S.
dc.contributor.author Treffers, E. E.
dc.contributor.author Bell, S.
dc.contributor.author Goodfellow, I.
dc.contributor.author Fang, Ying
dc.contributor.author Firth, A. E.
dc.contributor.author Snijder, E. J.
dc.contributor.author Brierley, I.
dc.date.accessioned 2017-02-15T14:42:50Z
dc.date.available 2017-02-15T14:42:50Z
dc.identifier.uri http://hdl.handle.net/2097/35166
dc.description Citation: Napthine, S., Treffers, E. E., Bell, S., Goodfellow, I., Fang, Y., Firth, A. E., . . . Brierley, I. (2016). A novel role for poly(C) binding proteins in programmed ribosomal frameshifting. Nucleic Acids Research, 44(12), 5491-5503. doi:10.1093/nar/gkw480
dc.description.abstract Translational control through programmed ribosomal frameshifting (PRF) is exploited widely by viruses and increasingly documented in cellular genes. Frameshifting is induced by mRNA secondary structures that compromise ribosome fidelity during decoding of a heptanucleotide 'slippery' sequence. The nsp2 PRF signal of porcine reproductive and respiratory syndrome virus is distinctive in directing both -2 and -1 PRF and in its requirement for a trans-acting protein factor, the viral replicase subunit nsp1 beta. Here we show that the the trans-activation of frameshifting is carried out by a protein complex composed of nsp1 beta and a cellular poly(C) binding protein (PCBP). From the results of in vitro translation and electrophoretic mobility shift assays, we demonstrate that a PCBP/nsp1 beta complex binds to a C-rich sequence downstream of the slippery sequence and here mimics the activity of a structured mRNA stimulator of PRF. This is the first description of a role for a trans-acting cellular protein in PRF. The discovery broadens the repertoire of activities associated with poly(C) binding proteins and prototypes a new class of virus-host interactions.
dc.relation.uri https://doi.org/10.1093/nar/gkw480
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Kh Domain Proteins
dc.subject Messenger-Rna
dc.subject Gene-Expression
dc.subject Poly(C)-Binding
dc.subject Proteins
dc.subject Translation Initiation
dc.title A novel role for poly(C) binding proteins in programmed ribosomal frameshifting
dc.type Article
dc.date.published 2016
dc.citation.doi 10.1093/nar/gkw480
dc.citation.epage 5503
dc.citation.issn 0305-1048
dc.citation.issue 12
dc.citation.jtitle Nucleic Acids Research
dc.citation.spage 5491
dc.citation.volume 44
dc.contributor.authoreid yfang
dc.contributor.kstate Fang, Ying


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