Genome sequence of Perigonia lusca single nucleopolyhedrovirus: insights into the evolution of a nucleotide metabolism enzyme in the family Baculoviridae

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dc.contributor.author Ardisson-Araujo, Daniel M. P.
dc.contributor.author Lima, R. N.
dc.contributor.author Melo, F. L.
dc.contributor.author Clem, R. J.
dc.contributor.author Huang, Ning
dc.contributor.author Bao, S. N.
dc.contributor.author Sosa-Gomez, D. R.
dc.contributor.author Ribeiro, B. M.
dc.date.accessioned 2017-02-14T23:08:48Z
dc.date.available 2017-02-14T23:08:48Z
dc.identifier.uri http://hdl.handle.net/2097/35147
dc.description Citation: Ardisson-Araujo, D. M. P., Lima, R. N., Melo, F. L., Clem, R. J., Huang, N., Bao, S. N., . . . Ribeiro, B. M. (2016). Genome sequence of Perigonia lusca single nucleopolyhedrovirus: insights into the evolution of a nucleotide metabolism enzyme in the family Baculoviridae. Scientific Reports, 6, 14. doi:10.1038/srep24612
dc.description.abstract The genome of a novel group II alphabaculovirus, Perigonia lusca single nucleopolyhedrovirus (PeluSNPV), was sequenced and shown to contain 132,831 bp with 145 putative ORFs (open reading frames) of at least 50 amino acids. An interesting feature of this novel genome was the presence of a putative nucleotide metabolism enzyme-encoding gene (pelu112). The pelu112 gene was predicted to encode a fusion of thymidylate kinase (tmk) and dUTP diphosphatase (dut). Phylogenetic analysis indicated that baculoviruses have independently acquired tmk and dut several times during their evolution. Two homologs of the tmk-dut fusion gene were separately introduced into the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) genome, which lacks tmk and dut. The recombinant baculoviruses produced viral DNA, virus progeny, and some viral proteins earlier during in vitro infection and the yields of viral occlusion bodies were increased 2.5-fold when compared to the parental virus. Interestingly, both enzymes appear to retain their active sites, based on separate modeling using previously solved crystal structures. We suggest that the retention of these tmk-dut fusion genes by certain baculoviruses could be related to accelerating virus replication and to protecting the virus genome from deleterious mutation.
dc.relation.uri https://doi.org/10.1038/srep24612
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Virus Dutpase
dc.subject Saccharomyces-Cerevisiae
dc.subject Replication
dc.subject Gene
dc.subject Identification
dc.subject Granulovirus
dc.title Genome sequence of Perigonia lusca single nucleopolyhedrovirus: insights into the evolution of a nucleotide metabolism enzyme in the family Baculoviridae
dc.type Article
dc.date.published 2016
dc.citation.doi 10.1038/srep24612
dc.citation.issn 2045-2322
dc.citation.jtitle Scientific Reports
dc.citation.spage 14
dc.citation.volume 6
dc.contributor.authoreid rclem
dc.contributor.kstate Clem, Rollie J.
dc.contributor.kstate Ardisson-Araujo, Daniel M. P.
dc.contributor.kstate Huang, Ning


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