Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo

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dc.contributor.author Girinathan, Brintha Parasumanna
dc.contributor.author Braun, S.
dc.contributor.author Sirigireddy, Apoorva Reddy Sirigireddy
dc.contributor.author Lopez, Jose Espinola
dc.contributor.author Govind, Revathi
dc.date.accessioned 2017-02-14T23:08:47Z
dc.date.available 2017-02-14T23:08:47Z
dc.identifier.uri http://hdl.handle.net/2097/35146
dc.description Citation: Girinathan, B. P., Braun, S., Sirigireddy, A. R., Lopez, J. E., & Govind, R. (2016). Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo. Plos One, 11(7), 18. doi:10.1371/journal.pone.0160107
dc.description.abstract Clostridium difficile is the principal cause of antibiotic-associated diarrhea. Major metabolic requirements for colonization and expansion of C. difficile after microbiota disturbance have not been fully determined. In this study, we show that glutamate utilization is important for C. difficile to establish itself in the animal gut. When the gluD gene, which codes for glutamate dehydrogenase (GDH), was disrupted, the mutant C. difficile was unable to colonize and cause disease in a hamster model. Further, from the complementation experiment it appears that extracellular GDH may be playing a role in promoting C. difficile colonization and disease progression. Quantification of free amino acids in the hamster gut during C. difficile infection showed that glutamate is among preferred amino acids utilized by C. difficile during its expansion. This study provides evidence of the importance of glutamate metabolism for C. difficile pathogenesis.
dc.relation.uri https://doi.org/10.1371/journal.pone.0160107
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Toxin Production
dc.subject Pseudomembranous Colitis
dc.subject Bacillus-Subtilis
dc.subject Metabolism
dc.subject Virulence
dc.subject Integration
dc.title Importance of Glutamate Dehydrogenase (GDH) in Clostridium difficile Colonization In Vivo
dc.type Article
dc.date.published 2016
dc.citation.doi 10.1371/journal.pone.0160107
dc.citation.issn 1932-6203
dc.citation.issue 7
dc.citation.jtitle Plos One
dc.citation.spage 18
dc.citation.volume 11
dc.contributor.authoreid rgovind
dc.contributor.kstate Govind, Revathi
dc.contributor.kstate Girinathan, Brintha Parasumanna


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