Superoxide dismutase 2 knockdown leads to defects in locomotor activity, sensitivity to paraquat, and increased cuticle pigmentation in Tribolium castaneum

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dc.contributor.author Tabunoki, Hiroko
dc.contributor.author Gorman, Maureen J.
dc.contributor.author Dittmer, Neal Thomas
dc.contributor.author Kanost, Michael R.
dc.date.accessioned 2017-02-14T23:03:05Z
dc.date.available 2017-02-14T23:03:05Z
dc.identifier.uri http://hdl.handle.net/2097/35134
dc.description Citation: Tabunoki, H., Gorman, M. J., Dittmer, N. T., & Kanost, M. R. (2016). Superoxide dismutase 2 knockdown leads to defects in locomotor activity, sensitivity to paraquat, and increased cuticle pigmentation in Tribolium castaneum. Scientific Reports, 6, 8. doi:10.1038/srep29583
dc.description.abstract Insects can rapidly adapt to environmental changes through physiological responses. The red flour beetle Tribolium castaneum is widely used as a model insect species. However, the stress-response system of this species remains unclear. Superoxide dismutase 2 (SOD2) is a crucial antioxidative enzyme that is found in mitochondria. T. castaneum SOD2 (TcSOD2) is composed of 215 amino acids, and has an iron/manganese superoxide dismutase domain. qRT-PCR experiments revealed that TcSOD2 was present through all developmental stages. To evaluate TcSOD2 function in T. castaneum, we performed RNAi and also assessed the phenotype and antioxidative tolerance of the knockdown of TcSOD2 by exposing larvae to paraquat. The administration of paraquat resulted in significantly higher 24-h mortality in TcSOD2 knockdown larval groups than in the control groups. The TcSOD2 knockdown adults moved significantly more slowly, had lower ATP content, and exhibited a different body color from the control groups. We found that TcSOD2 dsRNA treatment in larvae resulted in increased expression of tyrosinase and laccase2 mRNA after 10 days. This is the first report showing that TcSOD2 has an antioxidative function and demonstrates that T. castaneum may use an alternative antioxidative system when the SOD2-based system fails.
dc.relation.uri https://doi.org/10.1038/srep29583
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Bombyx-Mori
dc.subject Uric-Acid
dc.subject Life-Span
dc.subject Cocoon Shell
dc.subject Silkworm
dc.subject Drosophila
dc.title Superoxide dismutase 2 knockdown leads to defects in locomotor activity, sensitivity to paraquat, and increased cuticle pigmentation in Tribolium castaneum
dc.type Article
dc.date.published 2016
dc.citation.doi 10.1038/srep29583
dc.citation.issn 2045-2322
dc.citation.jtitle Scientific Reports
dc.citation.spage 8
dc.citation.volume 6
dc.contributor.authoreid mgorman
dc.contributor.authoreid ndittmer
dc.contributor.authoreid kanost
dc.contributor.kstate Gorman, Maureen J.
dc.contributor.kstate Dittmer, Neal Thomas
dc.contributor.kstate Kanost, Michael R.
dc.contributor.kstate Tabunoki, Hiroko


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