Sodium selectivity of Reissner’s membrane epithelial cells

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dc.contributor.author Yamazaki, M.
dc.contributor.author Kim, K. X.
dc.contributor.author Marcus, Daniel C.
dc.date.accessioned 2017-02-14T22:47:51Z
dc.date.available 2017-02-14T22:47:51Z
dc.identifier.uri http://hdl.handle.net/2097/35110
dc.description Citation: Yamazaki, M., Kim, K. X., & Marcus, D. C. (2011). Sodium selectivity of Reissner’s membrane epithelial cells. BMC Physiol, 11. doi:10.1186/1472-6793-11-4
dc.description.abstract Background: Sodium absorption by Reissner's membrane is thought to contribute to the homeostasis of the volume of cochlear endolymph. It was previously shown that the absorptive transepithelial current was blocked by amiloride and benzamil. The most commonly-observed target of these drugs is the epithelial sodium channel (ENaC), which is composed of the three subunits ?-,?- and ?-ENaC. However, other less-selective cation channels have also been observed to be sensitive to benzamil and amiloride. The aim of this study was to determine whether Reissner's membrane epithelial cells could support parasensory K+ absorption via amiloride- and benzamil-sensitive electrogenic pathways. Results: We determined the molecular and functional expression of candidate cation channels with gene array (GEO GSE6196), RT-PCR, and whole-cell patch clamp. Transcript expression analysis of Reissner's membrane detected no amiloride-sensitive acid-sensing ion channels (ASIC1a, ASIC2a, ASIC2b) nor amiloride-sensitive cyclic-nucleotide gated channels (CNGA1, CNGA2, CNGA4, CNGB3). By contrast, ?-,?- and ?-ENaC were all previously reported as present in Reissner's membrane. The selectivity of the benzamil-sensitive cation currents was observed in whole-cell patch clamp recordings under Cl--free conditions where cations were the only permeant species. The currents were carried by Na+ but not K+, and the permeability of Li+ was greater than that of Na+ in Reissner's membrane. Complete replacement of bath Na+ with the inpermeable cation NMDG+ led to the same inward current as with benzamil in a Na+ bath. Conclusions: These results are consistent with the amiloride/benzamil-sensitive absorptive flux of Reissner's membrane mediated by a highly Na+-selective channel that has several key characteristics in common with ???-ENaC. The amiloride-sensitive pathway therefore absorbs only Na+ in this epithelium and does not provide a parasensory K+ efflux route from scala media.
dc.relation.uri https://doi.org/10.1186/1472-6793-11-4
dc.rights Attribution 2.0 Generic (CC BY 2.0)
dc.rights.uri https://creativecommons.org/licenses/by/2.0/
dc.title Sodium selectivity of Reissner’s membrane epithelial cells
dc.type Article
dc.date.published 2011
dc.citation.doi 10.1186/1472-6793-11-4
dc.citation.issn 1472-6793
dc.citation.issue 4
dc.citation.jtitle BMC Physiology
dc.citation.volume 11
dc.contributor.authoreid marcus
dc.contributor.kstate Marcus, Daniel C.
dc.contributor.kstate Yamazaki, Muneharu
dc.contributor.kstate Kim, Kyunghee X.


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