Immobilization of ß-Galactosidase in Adsorptive Membranes for the Continuous Production of Galacto-Oligosaccharides from Lactose

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dc.contributor.author Engel, Larisa
dc.contributor.author Schneider, Philipp
dc.contributor.author Ebrahimi, Mehrdad
dc.contributor.author Czermak, Peter
dc.date.accessioned 2010-04-05T21:21:17Z
dc.date.available 2010-04-05T21:21:17Z
dc.date.issued 2010-04-05T21:21:17Z
dc.identifier.uri http://hdl.handle.net/2097/3508
dc.description.abstract A method for immobilization of ß-galactosidase from Kluyveromyces lactis on an adsorptive membrane for the continuous synthesis of galacto-oligosaccharides from lactose was carried out. The immobilization was performed at 4, 10, 15 and 40 °C. Two strongly basic anion exchange membrane with tradename MustangTMQ and SartobindTMQ were investigated. In static experiments, the highest enzyme activity was measured on MustangTMQ membranes at the immobilization temperature of 10 °C. The synthesis of GOS was performed in a Continuous Membrane-Chromatography-Reactor-System (CMCRS) at 40 °C and pH 7.0 using 20 wt% initial lactose concentration. The investigated membranes proved to be a good support for the continuous process at high convective flow rates in the enzyme reactor system. Up to 82 % lactose conversion with 24 % GOS yield was achieved at different fluxes. The corresponding reactor productivity for the production of GOS from lactose in the CCMRS was 98.7 grams GOS per hour and cubic centimeter membrane volume, which significantly exceeds previously reported results. en_US
dc.relation.uri http://www.bentham.org/open/tofsj/ en_US
dc.subject Immobilization en_US
dc.subject ß-galactosidase en_US
dc.subject Galacto-oligosaccharides en_US
dc.subject Adsorptive membrane en_US
dc.subject Chromatography membrane en_US
dc.subject Membrane reactor en_US
dc.title Immobilization of ß-Galactosidase in Adsorptive Membranes for the Continuous Production of Galacto-Oligosaccharides from Lactose en_US
dc.type Article (publisher version) en_US
dc.date.published 2007 en_US
dc.citation.doi 10.2174/1874256400701010017 en_US
dc.citation.epage 22 en_US
dc.citation.jtitle The Open Food Science Journal en_US
dc.citation.spage 17 en_US
dc.citation.volume 1 en_US
dc.contributor.authoreid pczermak en_US


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