Dynamics of protection against virulent challenge in swine vaccinated with attenuated African swine fever viruses

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dc.contributor.author Carlson, Jolene Christine
dc.date.accessioned 2016-09-28T13:22:17Z
dc.date.available 2016-09-28T13:22:17Z
dc.date.issued 2016-12-01 en_US
dc.identifier.uri http://hdl.handle.net/2097/34143
dc.description.abstract African swine fever (ASF) is a lethal hemorrhagic disease of swine caused by a double-stranded DNA virus. ASFV is endemic in Sardinia and Saharan Africa and has been recently expanded from the Caucasus to Eastern Europe. There is no vaccine to prevent the disease and current control measures are limited to culling and restricted animal movement. Swine infected with attenuated strains are protected against challenge with a homologous virulent virus, but there is limited knowledge of the host immune mechanisms generating that protection. Swine infected with Pret4 virus develop a fatal severe disease, while a derivative strain lacking virulence-associated gene 9GL (Pret4Δ9GL virus) is completely attenuated. Swine infected with Pret4 Δ9GL virus and challenged with the virulent parental virus at 7, 10, 14, 21, and 28 dpi showed a progressive acquisition of protection (from 40% at 7 dpi to 80% at 21 and 28 dpi). This animal model was used to associate the presence of host immune response and protection against the challenge. Anti-ASFV antibodies and cytokines in serum, as well as ASFV-specific IFN-γ production in PBMCs, were assessed in each group. Interestingly, with the exception of ASFV-specific antibodies in the surviving swine challenged at 21 and 28 dpi, no solid association between any of the parameters assessed and the extent of protection could be established. These results were corroborated using a similar model based on the use of a rationally attenuated derivative of the highly virulent strain Georgia 2007. These results, encompassing data from 114 immunized swine, underscore the complexity of the system under study where it is very plausible that protection against disease or infection relies heavily on the concurrence and or interaction of different host immune mechanisms. en_US
dc.description.sponsorship The State of Kansas National Bio and Agro-Defense Facility Fund (NBAF), an interagency agreement with the Science and Technology Directorate of the U.S. Department of Homeland Security (DHS) under Award Numbers HSHQDC-11-X-00077 and HSHQPM-12-X-00005, and the National Center for Foreign Animal and Zoonotic Disease Defense (ZADD) Fellows Career Development Program. The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of DHS. en_US
dc.language.iso en_US en_US
dc.publisher Kansas State University en
dc.subject African swine fever virus en_US
dc.subject Interferon gamma en_US
dc.subject Attenuated virus en_US
dc.subject Correlates of protection en_US
dc.subject Vaccination en_US
dc.subject 9GL en_US
dc.title Dynamics of protection against virulent challenge in swine vaccinated with attenuated African swine fever viruses en_US
dc.type Dissertation en_US
dc.description.degree Doctor of Philosophy en_US
dc.description.level Doctoral en_US
dc.description.department Department of Diagnostic Medicine/Pathobiology en_US
dc.description.advisor Manuel Borca en_US
dc.description.advisor Stephen Higgs en_US
dc.date.published 2016 en_US
dc.date.graduationmonth December en_US

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