Standardizing Umbilical Cord Mesenchymal Stromal Cells for Translation to Clinical Use: Selection of GMP-Compliant Medium and a Simplified Isolation Method

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dc.contributor.author Smith, J. Robert
dc.contributor.author Pfeifer, Kyle
dc.contributor.author Petry, Florian
dc.contributor.author Powell, Natalie
dc.contributor.author Delzeit, Jennifer
dc.contributor.author Weiss, Mark L.
dc.date.accessioned 2016-09-20T17:37:31Z
dc.date.available 2016-09-20T17:37:31Z
dc.date.issued 2016-02-04
dc.identifier.uri http://hdl.handle.net/2097/34089
dc.description Citation: Smith, J. R., Pfeifer, K., Petry, F., Powell, N., Delzeit, J., & Weiss, M. L. (2016). Standardizing Umbilical Cord Mesenchymal Stromal Cells for Translation to Clinical Use: Selection of GMP-Compliant Medium and a Simplified Isolation Method. Stem Cells International, 14. doi:10.1155/2016/6810980
dc.description.abstract Umbilical cord derived mesenchymal stromal cells (UC-MSCs) are a focus for clinical translation but standardized methods for isolation and expansion are lacking. Previously we published isolation and expansion methods for UC-MSCs which presented challenges when considering good manufacturing practices (GMP) for clinical translation. Here, a new and more standardized method for isolation and expansion of UC-MSCs is described. The new method eliminates dissection of blood vessels and uses a closed-vessel dissociation following enzymatic digestion which reduces contamination risk and manipulation time. The new method produced >10 times more cells per cm of UC than our previous method. When biographical variables were compared, more UC-MSCs per gram were isolated after vaginal birth compared to Caesarian-section births, an unexpected result. UC-MSCs were expanded in medium enriched with 2%, 5%, or 10% pooled human platelet lysate (HPL) eliminating the xenogeneic serum components. When the HPL concentrations were compared, media supplemented with 10% HPL had the highest growth rate, smallest cells, and the most viable cells at passage. UC-MSCs grown in 10% HPL had surface marker expression typical of MSCs, high colony forming efficiency, and could undergo trilineage differentiation. The new protocol standardizes manufacturing of UC-MSCs and enables clinical translation.
dc.relation.uri https://doi.org/10.1155/2016/6810980
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Human Platelet Lysate
dc.subject Jelly-Derived Cells
dc.subject Fetal Bovine Serum
dc.subject Stem-Cells
dc.subject Bone-Marrow
dc.subject Msc
dc.title Standardizing Umbilical Cord Mesenchymal Stromal Cells for Translation to Clinical Use: Selection of GMP-Compliant Medium and a Simplified Isolation Method
dc.type Article
dc.date.published 2016
dc.citation.doi 10.1155/2016/6810980
dc.citation.issn 1687-966X
dc.citation.jtitle Stem Cells International
dc.citation.spage 14
dc.contributor.authoreid mlweiss


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