Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae

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dc.contributor.author Xia, P. P.
dc.contributor.author Wang, Y. T.
dc.contributor.author Zhu, C. R.
dc.contributor.author Zou, Y. J.
dc.contributor.author Yang, Y.
dc.contributor.author Liu, W.
dc.contributor.author Hardwidge, Philip R.
dc.contributor.author Zhu, G. Q.
dc.date.accessioned 2016-09-20T17:34:06Z
dc.date.available 2016-09-20T17:34:06Z
dc.date.issued 2016-02-09
dc.identifier.uri http://hdl.handle.net/2097/34068
dc.description Citation: Xia, P. P., Wang, Y. T., Zhu, C. R., Zou, Y. J., Yang, Y., Liu, W., . . . Zhu, G. Q. (2016). Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae. Veterinary Research, 47, 7. doi:10.1186/s13567-016-0313-5
dc.description.abstract F4(+) enterotoxigenic Escherichia coli (ETEC) strains cause diarrheal disease in neonatal and post-weaned piglets. Several different host receptors for F4 fimbriae have been described, with porcine aminopeptidase N (APN) reported most recently. The FaeG subunit is essential for the binding of the three F4 variants to host cells. Here we show in both yeast two-hybrid and pulldown assays that APN binds directly to FaeG, the major subunit of F4 fimbriae, from three serotypes of F4(+) ETEC. Modulating APN gene expression in IPEC-J2 cells affected ETEC adherence. Antibodies raised against APN or F4 fimbriae both reduced ETEC adherence. Thus, APN mediates the attachment of F4(+) E. coli to intestinal epithelial cells.
dc.relation.uri https://doi.org/10.1186/s13567-016-0313-5
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject F4 Fimbriae
dc.subject Brush-Borders
dc.subject In-Vitro
dc.subject Adhesin Variants
dc.subject Receptor-Binding
dc.subject Epithelial-Cell
dc.title Porcine aminopeptidase N binds to F4(+) enterotoxigenic Escherichia coli fimbriae
dc.type Article
dc.date.published 2016
dc.citation.doi 10.1186/s13567-016-0313-5
dc.citation.issn 2151-0032
dc.citation.jtitle Veterinary Research
dc.citation.spage 7
dc.citation.volume 47
dc.contributor.authoreid philiphardwidge


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