Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection

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dc.contributor.author Udukala, D. N.
dc.contributor.author Wang, H. W.
dc.contributor.author Wendel, S. O.
dc.contributor.author Malalasekera, A. P.
dc.contributor.author Samarakoon, Thilani Nishanthika
dc.contributor.author Yapa, A. S.
dc.contributor.author Abayaweera, G.
dc.contributor.author Basel, M. T.
dc.contributor.author Maynez, P.
dc.contributor.author Li, Ping
dc.contributor.author Higgins, Daniel A.
dc.contributor.author Gadbury, Gary
dc.contributor.author Troyer, Deryl L.
dc.contributor.author Bossmann, Stefan H.
dc.date.accessioned 2016-09-20T17:02:05Z
dc.date.available 2016-09-20T17:02:05Z
dc.identifier.uri http://hdl.handle.net/2097/33976
dc.description Citation: Udukala, D. N., Wang, H. W., Wendel, S. O., Malalasekera, A. P., Samarakoon, T. N., Yapa, A. S., . . . Bossmann, S. H. (2016). Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection. Beilstein Journal of Nanotechnology, 7, 364-373. doi:10.3762/bjnano.7.33
dc.description Additional Authors: Ortega, R.;Toledo, Y.;Bossmann, L.;Robinson, C.;Janik, K. E.;Koper, O. B.;Motamedi, M.;Zhu, G. H.
dc.description.abstract Proteases, including matrix metalloproteinases (MMPs), tissue serine proteases, and cathepsins (CTS) exhibit numerous functions in tumor biology. Solid tumors are characterized by changes in protease expression levels by tumor and surrounding tissue. Therefore, monitoring protease levels in tissue samples and liquid biopsies is a vital strategy for early cancer detection. Water-dispersable Fe/Fe3O4-core/shell based nanoplatforms for protease detection are capable of detecting protease activity down to sub-femtomolar limits of detection. They feature one dye (tetrakis(carboxyphenyl) porphyrin (TCPP)) that is tethered to the central nanoparticle by means of a protease-cleavable consensus sequence and a second dye (Cy 5.5) that is directly linked. Based on the protease activities of urokinase plasminogen activator (uPA), MMPs 1, 2, 3, 7, 9, and 13, as well as CTS B and L, human breast cancer can be detected at stage I by means of a simple serum test. By monitoring CTS B and L stage 0 detection may be achieved. This initial study, comprised of 46 breast cancer patients and 20 apparently healthy human subjects, demonstrates the feasibility of protease-activity-based liquid biopsies for early cancer diagnosis.
dc.relation.uri https://doi.org/10.3762/bjnano.7.33
dc.rights Attribution 2.0 Generic (CC BY 2.0)
dc.rights.uri https://creativecommons.org/licenses/by/2.0/
dc.subject Biophotonics
dc.subject Breast Cancer
dc.subject Iron/Iron Oxide Nanoparticle
dc.subject Liquid
dc.subject Biopsy
dc.subject Nanodiagnostics Detection
dc.title Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection
dc.type Article
dc.date.published 2016
dc.citation.doi 10.3762/bjnano.7.33
dc.citation.epage 373
dc.citation.issn 2190-4286
dc.citation.jtitle Beilstein Journal of Nanotechnology
dc.citation.spage 364
dc.citation.volume 7
dc.contributor.authoreid pli
dc.contributor.authoreid higgins
dc.contributor.authoreid gadbury
dc.contributor.authoreid troyer
dc.contributor.authoreid sbossman


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