mTORC1 and CK2 coordinate ternary and eIF4F complex assembly

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dc.contributor.author Gandin, V.
dc.contributor.author Masvidal, L.
dc.contributor.author Cargnello, M.
dc.contributor.author Gyenis, L.
dc.contributor.author McLaughlan, S.
dc.contributor.author Cai, Y. T.
dc.contributor.author Tenkerian, C.
dc.contributor.author Morita, M.
dc.contributor.author Balanathan, P.
dc.contributor.author Jean-Jean, O.
dc.contributor.author Stambolic, V.
dc.contributor.author Trost, M.
dc.contributor.author Furic, L.
dc.contributor.author Asano, Katsura
dc.date.accessioned 2016-09-20T14:53:40Z
dc.date.available 2016-09-20T14:53:40Z
dc.identifier.uri http://hdl.handle.net/2097/33948
dc.description Citation: Gandin, V., Masvidal, L., Cargnello, M., Gyenis, L., McLaughlan, S., Cai, Y. T., . . . Topisirovic, I. (2016). mTORC1 and CK2 coordinate ternary and eIF4F complex assembly. Nature Communications, 7, 15. doi:10.1038/ncomms11127
dc.description Additional Authors: Larose, L.;Koromilas, A. E.;Litchfield, D.;Larsson, O.;Topisirovic, I.
dc.description.abstract Ternary complex (TC) and eIF4F complex assembly are the two major rate-limiting steps in translation initiation regulated by eIF2 alpha phosphorylation and the mTOR/4E-BP pathway, respectively. How TC and eIF4F assembly are coordinated, however, remains largely unknown. We show that mTOR suppresses translation of mRNAs activated under short-term stress wherein TC recycling is attenuated by eIF2 alpha phosphorylation. During acute nutrient or growth factor stimulation, mTORC1 induces eIF2 beta phosphorylation and recruitment of NCK1 to eIF2, decreases eIF2 alpha phosphorylation and bolsters TC recycling. Accordingly, eIF2 beta mediates the effect of mTORC1 on protein synthesis and proliferation. In addition, we demonstrate a formerly undocumented role for CK2 in regulation of translation initiation, whereby CK2 stimulates phosphorylation of eIF2 beta and simultaneously bolsters eIF4F complex assembly via the mTORC1/4E-BP pathway. These findings imply a previously unrecognized mode of translation regulation, whereby mTORC1 and CK2 coordinate TC and eIF4F complex assembly to stimulate cell proliferation.
dc.relation.uri https://doi.org/10.1038/ncomms11127
dc.rights Attribution 4.0 International (CC BY 4.0)
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject Messenger-Rna Translation
dc.subject Endoplasmic-Reticulum Stress
dc.subject Initiation-Factor Eif2-Alpha
dc.subject Unfolded Protein Response
dc.subject Tumor-Suppressor Pten
dc.subject Beta-Subunit
dc.title mTORC1 and CK2 coordinate ternary and eIF4F complex assembly
dc.type Article
dc.date.published 2016
dc.citation.doi 10.1038/ncomms11127
dc.citation.issn 2041-1723
dc.citation.jtitle Nature Communications
dc.citation.spage 15
dc.citation.volume 7
dc.contributor.authoreid kasano


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Attribution 4.0 International (CC BY 4.0) Except where otherwise noted, the use of this item is bound by the following: Attribution 4.0 International (CC BY 4.0)

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